LOCUS       sequence-305164-       11619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses nCDA1-Delta-192-BE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Engineering of high-precision base editors for site-specific single 
            nucleotide replacement.
  JOURNAL   Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.
  PUBMED    30683865
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-25"; volume: "10(1):439. doi"; pages: " 
            10.1038/s41467-018-08034-8"
COMMENT     Sequence Label: pJT79_GalL_nCDA1-Delta-192-BE3
FEATURES             Location/Qualifiers
     source          1..11619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1439..1462
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1483..1499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1483..1499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(2089..2115,2116..2118,2119..6192)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2089..6192
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6205..6453
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6466..6486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6502..6749
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6518..6536)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6767..6786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6768..6786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6796..6813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6796..6812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6805..6827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6953..7408
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7040..7059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7250..7271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7709..8116
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8117..9211
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9705..9724)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9824..9846
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9884..9902)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9944..10447
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10484..10588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10589..10657,10658..11449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10807..10826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgt gacagccctc cgaaggaaga ctctcctccg
     1081 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     1141 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     1201 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     1261 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     1321 tctattaacg gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     1381 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     1441 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
     1501 cccgggaaaa aaatgaccga cgctgagtac gtgagaatcc atgagaagtt ggacatctac
     1561 acgtttaaga aacagttttt caacaacaaa aaatccgtgt cgcatagatg ctacgttctc
     1621 tttgaattaa aacgacgggg tgaacgtaga gcgtgttttt ggggctatgc tgtgaataaa
     1681 ccacagagcg ggacagaacg tggcattcac gccgaaatct ttagcattag aaaagtcgaa
     1741 gaatacctgc gcgacaaccc cggacaattc acgataaatt ggtactcatc ctggagtcct
     1801 tgtgcagatt gcgctgaaaa gatcttagaa tggtataacc aggagctgcg ggggaacggc
     1861 cacactttga aaatctgggc ttgcaaactc tattacgaga aaaatgcgag gaatcaaatt
     1921 gggctgtgga acctcagaga taacggggtt gggttgaatg taatggtaag tgaacactac
     1981 caatgttgca ggaaaatatt catccaatcg tcgcacaatc aattgaatga gaatagatgg
     2041 cttgagaaga ctttgaagcg agctgaaaaa cgacggagcg agttgtccat ggacaagaag
     2101 tactccattg ggctcgctat cggcacaaac agcgtcggtt gggccgtcat tacggacgag
     2161 tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata ccgatcgcca cagcataaag
     2221 aagaacctca ttggcgccct cctgttcgac tccggggaga cggccgaagc cacgcggctc
     2281 aaaagaacag cacggcgcag atatacccgc agaaagaatc ggatctgcta cctgcaggag
     2341 atctttagta atgagatggc taaggtggat gactctttct tccataggct ggaggagtcc
     2401 tttttggtgg aggaggataa aaagcacgag cgccacccaa tctttggcaa tatcgtggac
     2461 gaggtggcgt accatgaaaa gtacccaacc atatatcatc tgaggaagaa gcttgtagac
     2521 agtactgata aggctgactt gcggttgatc tatctcgcgc tggcgcatat gatcaaattt
     2581 cggggacact tcctcatcga gggggacctg aacccagaca acagcgatgt cgacaaactc
     2641 tttatccaac tggttcagac ttacaatcag cttttcgaag agaacccgat caacgcatcc
     2701 ggagttgacg ccaaagcaat cctgagcgct aggctgtcca aatcccggcg gctcgaaaac
     2761 ctcatcgcac agctccctgg ggagaagaag aacggcctgt ttggtaatct tatcgccctg
     2821 tcactcgggc tgacccccaa ctttaaatct aacttcgacc tggccgaaga tgccaagctt
     2881 caactgagca aagacaccta cgatgatgat ctcgacaatc tgctggccca gatcggcgac
     2941 cagtacgcag accttttttt ggcggcaaag aacctgtcag acgccattct gctgagtgat
     3001 attctgcgag tgaacacgga gatcaccaaa gctccgctga gcgctagtat gatcaagcgc
     3061 tatgatgagc accaccaaga cttgactttg ctgaaggccc ttgtcagaca gcaactgcct
     3121 gagaagtaca aggaaatttt cttcgatcag tctaaaaatg gctacgccgg atacattgac
     3181 ggcggagcaa gccaggagga attttacaaa tttattaagc ccatcttgga aaaaatggac
     3241 ggcaccgagg agctgctggt aaagcttaac agagaagatc tgttgcgcaa acagcgcact
     3301 ttcgacaatg gaagcatccc ccaccagatt cacctgggcg aactgcacgc tatcctcagg
     3361 cggcaagagg atttctaccc ctttttgaaa gataacaggg aaaagattga gaaaatcctc
     3421 acatttcgga taccctacta tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg
     3481 atgactcgca aatcagaaga gaccatcact ccctggaact tcgaggaagt cgtggataag
     3541 ggggcctctg cccagtcctt catcgaaagg atgactaact ttgataaaaa tctgcctaac
     3601 gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact tcacagttta taacgagctc
     3661 accaaggtca aatacgtcac agaagggatg agaaagccag cattcctgtc tggagagcag
     3721 aagaaagcta tcgtggacct cctcttcaag acgaaccgga aagttaccgt gaaacagctc
     3781 aaagaagact atttcaaaaa gattgaatgt ttcgactctg ttgaaatcag cggagtggag
     3841 gatcgcttca acgcatccct gggaacgtat cacgatctcc tgaaaatcat taaagacaag
     3901 gacttcctgg acaatgagga gaacgaggac attcttgagg acattgtcct cacccttacg
     3961 ttgtttgaag atagggagat gattgaagaa cgcttgaaaa cttacgctca tctcttcgac
     4021 gacaaagtca tgaaacagct caagaggcgc cgatatacag gatgggggcg gctgtcaaga
     4081 aaactgatca atgggatccg agacaagcag agtggaaaga caatcctgga ttttcttaag
     4141 tccgatggat ttgccaaccg gaacttcatg cagttgatcc atgatgactc tctcaccttt
     4201 aaggaggaca tccagaaagc acaagtttct ggccaggggg acagtcttca cgagcacatc
     4261 gctaatcttg caggtagccc agctatcaaa aagggaatac tgcagaccgt taaggtcgtg
     4321 gatgaactcg tcaaagtaat gggaaggcat aagcccgaga atatcgttat cgagatggcc
     4381 cgagagaacc aaactaccca gaagggacag aagaacagta gggaaaggat gaagaggatt
     4441 gaagagggta taaaagaact ggggtcccaa atccttaagg aacacccagt tgaaaacacc
     4501 cagcttcaga atgagaagct ctacctgtac tacctgcaga acggcaggga catgtacgtg
     4561 gatcaggaac tggacatcaa tcggctctcc gactacgacg tggatcatat cgtgccccag
     4621 tcttttctca aagatgattc tattgataat aaagtgttga caagatccga taaaaataga
     4681 gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg
     4741 cagctgctga acgccaaact gatcacacaa cggaagttcg ataatctgac taaggctgaa
     4801 cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca aaaggcagct tgttgagaca
     4861 cgccagatca ccaagcacgt ggcccaaatt ctcgattcac gcatgaacac caagtacgat
     4921 gaaaatgaca aactgattcg agaggtgaaa gttattactc tgaagtctaa gctggtctca
     4981 gatttcagaa aggactttca gttttataag gtgagagaga tcaacaatta ccaccatgcg
     5041 catgatgcct acctgaatgc agtggtaggc actgcactta tcaaaaaata tcccaagctt
     5101 gaatctgaat ttgtttacgg agactataaa gtgtacgatg ttaggaaaat gatcgcaaag
     5161 tctgagcagg aaataggcaa ggccaccgct aagtacttct tttacagcaa tattatgaat
     5221 tttttcaaga ccgagattac actggccaat ggagagattc ggaagcgacc acttatcgaa
     5281 acaaacggag aaacaggaga aatcgtgtgg gacaagggta gggatttcgc gacagtccgg
     5341 aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc
     5401 ttctccaagg aaagtatcct cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa
     5461 gattgggacc ccaagaaata cggcggattc gattctccta cagtcgctta cagtgtactg
     5521 gttgtggcca aagtggagaa agggaagtct aaaaaactca aaagcgtcaa ggaactgctg
     5581 ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg
     5641 aaaggatata aagaggtcaa aaaagacctc atcattaagc ttcccaagta ctctctcttt
     5701 gagcttgaaa acggccggaa acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac
     5761 gagctggcac tgccctctaa atacgttaat ttcttgtatc tggccagcca ctatgaaaag
     5821 ctcaaagggt ctcccgaaga taatgagcag aagcagctgt tcgtggaaca acacaaacac
     5881 taccttgatg agatcatcga gcaaataagc gaattctcca aaagagtgat cctcgccgac
     5941 gctaacctcg ataaggtgct ttctgcttac aataagcaca gggataagcc catcagggag
     6001 caggcagaaa acattatcca cttgtttact ctgaccaact tgggcgcgcc tgcagccttc
     6061 aagtacttcg acaccaccat agacagaaag cggtacacct ctacaaagga ggtcctggac
     6121 gccacactga ttcatcagtc aattacgggg ctctatgaaa caagaatcga cctctctcag
     6181 ctcggtggag actctggtgg ttcaactaat ttatctgata ttattgaaaa agaaacaggt
     6241 aaacaattag ttattcaaga atcaatttta atgttgccag aagaagttga agaagttatt
     6301 ggtaataaac cagaatctga tattttagtt catactgctt atgatgaatc tactgatgaa
     6361 aatgttatgt tattgacttc agatgctcca gaatataaac catgggcttt agttattcaa
     6421 gattctaatg gtgaaaataa aattaaaatg ttgtctggtg gttcacccaa gaagaagagg
     6481 aaggtgtgat ctcttctcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     6541 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     6601 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     6661 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     6721 ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct atagtgagtc
     6781 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     6961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7381 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     7441 gcggtatttc acaccgcata tcgaccggtc gaggagaact tctagtatat ctacatacct
     7501 aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt
     7561 caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg
     7621 ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc
     7681 tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc
     7741 aagagttcga atctcttagc aaccattatt tttttcctca acataacgag aacacacagg
     7801 ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg
     7861 cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc
     7921 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
     7981 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
     8041 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatatttca
     8101 aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt
     8161 tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc
     8221 caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg
     8281 tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg
     8341 tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa
     8401 aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc
     8461 tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt
     8521 cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt
     8581 cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc
     8641 tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt
     8701 tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc
     8761 tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc
     8821 aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga
     8881 agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc
     8941 agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc
     9001 aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc
     9061 attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc
     9121 aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt
     9181 cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta
     9241 cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg
     9301 ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa
     9361 ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa
     9421 aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca aaaagttagg
     9481 tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat tttctctaaa
     9541 aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag
     9601 tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg
     9661 tcagaaacgg ccttaacgac gtagtcgata tggtgcactc tcagtacaat ctgctctgat
     9721 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     9781 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     9841 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     9901 tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct tgcctgtaac
     9961 ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt
    10021 tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg
    10081 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat
    10141 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta
    10201 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc
    10261 ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc
    10321 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
    10381 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
    10441 acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    10561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    10621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    10681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10801 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    10861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    11041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    11101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    11581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
