LOCUS       sequence-305175-        3189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BRET biosensor for detection of G-alpha-i-GTP in mammalian 
            expression vector pcDNA3.1(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Maziarz M, Park JC, Leyme A, Marivin A, Garcia-Lopez A, Patel PP, 
            Garcia-Marcos M
  TITLE     Revealing the Activity of Trimeric G-proteins in Live Cells with a 
            Versatile Biosensor Design.
  JOURNAL   Cell. 2020 Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020.
  PUBMED    32634377
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 27. pii: S0092-8674(20)30752-2. doi: 
            10.1016/j.cell.2020.06.020."
COMMENT     Sequence Label: pcDNA3.1-G-alpha-i*-BERKY3
FEATURES             Location/Qualifiers
     source          1..3189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..588
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             613..633
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1354..1605
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2086..2337
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             2815..3066
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3157..3186
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgggatgta taaaatcaaa agggaaagac agcaagcttg gatcaggcgg tagcggggga
       61 tcaggcggta gcgggatggt cttcacactc gaagatttcg ttggggactg gcgacagaca
      121 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
      181 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
      241 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
      301 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
      361 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
      421 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
      481 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
      541 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga attcggatct
      601 ggtggttcag gcgaaaacct gtattttcag ggcggaagcg gagaagagga ggaaaagaaa
      661 aaacaacagg aggaggaggc ggagaggctg aggaggatac aagaggaaat ggaaaaagaa
      721 agaaagcggc gagaggaaga tgagcagcga cgccgcaagg aggaggaaga gagaaggatg
      781 aaattggaga tggaggcaaa acgcaaacaa gaagaggaag aacggaagaa acgggaggat
      841 gatgaaaagc ggaagaaaaa gggatctgga gggtcagggg gcgcgcccat ggtttcaaag
      901 ggagaagagt tgttcacagg ggttgtaccg attcttgtag aacttgacgg agatgtcaac
      961 gggcacaagt ttagtgtaag tggagaaggc gagggcgatg cgacctatgg aaaactgacc
     1021 cttaaattta tttgtactac gggtaaattg cccgttccct ggccgacatt ggtaactaca
     1081 ttcggatatg gtttgatgtg ctttgcaagg tatccggatc acatgaagca gcatgatttc
     1141 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1201 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1261 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1321 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1381 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1441 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1501 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1561 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgc gcccggagga
     1621 atggtttcaa agggagaaga gttgttcaca ggggttgtac cgattcttgt agaacttgac
     1681 ggagatgtca acgggcacaa gtttagtgta agtggagaag gcgagggcga tgcgacctat
     1741 ggaaaactga cccttaaatt tatttgtact acgggtaaat tgcccgttcc ctggccgaca
     1801 ttggtaacta cattcggata tggtttgatg tgctttgcaa ggtatccgga tcacatgaag
     1861 cagcatgatt tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1921 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1981 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2041 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2101 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2161 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2221 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2281 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
     2341 tccggaggaa tggtttcaaa gggagaagag ttgttcacag gggttgtacc gattcttgta
     2401 gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa gtggagaagg cgagggcgat
     2461 gcgacctatg gaaaactgac ccttaaattt atttgtacta cgggtaaatt gcccgttccc
     2521 tggccgacat tggtaactac attcggatat ggtttgatgt gctttgcaag gtatccggat
     2581 cacatgaagc agcatgattt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2641 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2701 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2761 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2821 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2881 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2941 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3001 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3061 tacaagggat ccggtggcac cggtggatca ggctcttctc gaggttacta ccatggtatt
     3121 tgggtgggtg aagaaggtcg actttctcga ggtggagaac aaaaacttat ttctgaagaa
     3181 gatctgtga
//
