LOCUS       sequence-305176-       11416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttling cassette containing the Tol ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pF3-CAG-Tol-FRT
FEATURES             Location/Qualifiers
     source          1..11416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(78..182)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    230..263
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     enhancer        298..677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        679..955
                     /label=chicken beta-actin promoter
     intron          956..1971
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1895..1917
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1979..1998
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2026..2035
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9361..9387
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9416..9433)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9422..9629
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(9635..9682)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9703..9720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9874..10462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9954..9973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10633..11416,1..8,9..77))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11256..11275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
       61 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      181 cgcacatttc cccgaaaagt gccacctatt taaatgaagt tcctattccg aagttcctat
      241 tcttcaaata gtataggaac ttcttaatta acgatgtacg ggccagatat acgcgttgac
      301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      661 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
      721 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
      781 gggcgggggg gggggggggc gcgcgccrgg gggggsgggg sggggsgrgg ggcggggsgg
      841 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      901 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      961 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     1021 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     1081 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     1141 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     1201 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     1261 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     1321 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1381 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     1441 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     1501 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1561 cctcgggccg gggrgggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     1621 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1681 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     1741 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1801 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1861 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1921 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1981 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgccac catgggcaac
     2041 agagccttca ctaaatatcc agcagacatc caggactact tcaagcagac ctttccagag
     2101 gggtatcatt gggagaggtc tatgacctac gaggatcagg ggatttgcac agccacttcc
     2161 aacatctcta tgcgcggcga ctgtttcttt tatgacatcc gatttgatgg tgtgaacttc
     2221 ccccctaatg gcccagtcat gcagaagaaa acactgaaat gggagcccag cactgaaaag
     2281 atgtacgtga gagatggagt cctgaagggg gacgtgaata tggcactgct cctggaggga
     2341 ggaggacact accgatgcga ctttaagacc acatataaag ccaagaaaga cgtgcgactg
     2401 ccagattacc acttcgtcga ccataggatc gagattctca agcatgacaa agattacaac
     2461 aaggtgaaac tgtatgaaaa tgctgtcgca agatattcca tgctcccttc tcaggcaaaa
     2521 ggaaaccggg tgttcgccaa gtacccagac cacatccccg attatttcaa gcagagcttt
     2581 ccaaaagggt tcagttggga gagatcactg atgtttgaag atggtggcgt gtgcattgcc
     2641 actaatgata tcaccctgaa aggcgacacc ttctttaaca aggtgcggtt tgacggagtc
     2701 aacttcccac ccaatgggcc cgtgatgcag aagaaaaccc tgaaatggga ggcttccaca
     2761 gaaaagatgt atctcagaga tggtgtgctg acaggcgaca tcactatggc actcctgctc
     2821 aagggcgatg tgcactacag atgcgacttt cggactacct ataaatctag gcaggagggt
     2881 gtgaagctgc caggctatca tttcgtcgac cattgtatca gtattctgag acacgacaag
     2941 gattacaacg aggtgaaact ctatgaacac gccgtcgctc attcaggtct gcccgataac
     3001 gtgaagggca gcaaaacctt cattaatcac acacagggaa tccctgactt ctttaagcag
     3061 agttttccag aggggttcac atgggaaaga atcacaactt acgaggatgg aggggtgctg
     3121 accgctacac aggacacttc actccagaac ggctgcctga tctacaatgt gaagatcaac
     3181 ggagtcaatt tccctagtaa cgggccagtg atgcagaaga aaactctggg atgggaggcc
     3241 tcaaccgaaa tgctctatcc tgctgactct ggactgcggg ggcacagtca gatggccctg
     3301 aaactcgtgg gtggcggata cctccattgc agcctgaaga ccacatatcg ctccaagaaa
     3361 cccgccaaga acctgaaaat gcctggcttc tactttgtgg ataggagact ggagaggatc
     3421 aaggaagctg acaaagagac atacgtggag cagcacgaaa tggcagtcgc cagatattgt
     3481 gacctcccct caaagctggg tcacagcggc catcgggtgt tcaccaaata ccccgaggaa
     3541 attcctgatt acttcaagca ggccttccct gagggcctca gctgggaacg ctccctggag
     3601 tttgaagacg ggggtagcgc atccgtgtct gcccacatca gcctgcgagg aaacacattc
     3661 taccataagt ccaagttcac cggtgtcaat ttcccagcag atggccccat tatgcagaac
     3721 cagtcagtgg actgggaacc cagcaccgag aagatcacag cctccgatgg agtgctgaaa
     3781 ggcgacgtga ccatgtacct gaagctcgag ggcggaggga accacaagtg ccagatgaaa
     3841 actacctaca aggccgctaa agagattctg gaaatgcctg gagaccacta tatcgggcat
     3901 cggctggtgc gcaagactga gggcaacatc accgagcagg tggaagatgc tgtcgcacac
     3961 agtggatcaa aaacattcat taaccatact caggggatcc ccgacttctt taagcagagc
     4021 tttcctgagg gcttcacctg ggaaagagtg acaacttacg aggacggtgg cgtcctgact
     4081 gccacccagg atacatccct ccaggacgga tgtctgatct acaacgtgaa gatccgggga
     4141 gtcaacttta cttctaatgg gcctgtgatg cagaagaaaa ccctgggatg ggaagcattc
     4201 acagagactc tctacccagc cgatggaggg ctggagggga gaaatgacat ggccctgaaa
     4261 ctggtcggtg gctctcacct gatcgctaat attaagacca cataccggag taagaaacca
     4321 gctaagaacc tgaaaatgcc cggcgtgtac tatgtcgatt atcgactgga aaggatcaag
     4381 gaggcaaaca atgaaaccta tgtcgagcag cacgaagtgg ccgtcgctcg gtattgcgac
     4441 ctcccctcca agctgggtca taaactcaac ggcgtgcagt gtttctctcg ctaccctgat
     4501 cacatgaagc agcatgactt ctttaaatcc gctatgccag agggctacgt gcaggaacgc
     4561 acaattttct ttaaggacga tggaaactac aaaacacgag ccgaggtgaa gtttgaagga
     4621 gatactctcg tcaataggat tgagctgaag gggatcgact tcaaagaaga tggtaacatc
     4681 ctgggccaca agctcgagta caactataat tcccataacg tgtacattat ggctgacaag
     4741 cagaaaaacg ggatcaaggt caatttcaaa attcgccaca acatcgagga tggctctgtg
     4801 cagctggccg accattacca gcagaatacc ccaatcggcg acggacctgt gctgctccca
     4861 gacaaccact atctcagtac acagtcagcc ctgagcaagg atcccaatga gaaaagggac
     4921 catatggtgc tgctcgaatt cgtcaccgca gccggaatta cactcgggat ggatgagctg
     4981 tacaagctac caggcatccg gcagggctat ggcctcacag agacaaccag cgccatcctg
     5041 atcacaccag aaggcgacga taaaccagga gcagtgggca aggtggtccc tttcttcgag
     5101 gccaaggtgg tcgatctgga cacaggaaag acacttggcg tcaatcagag gggggaactg
     5161 tgcgtgaggg gccccatgat tatgagcgga tacgtgaata atccagaagc cactaatgcc
     5221 cttattgata aagacggatg gctgcattct ggagacatcg catattggga tgaggatgag
     5281 cattttttta tcgtcgatcg cctgaagagc ttgattaagt ataagggcta ccaggtggcc
     5341 cctgcagaac tcgagtccat cctcctccaa caccctaaca tcttcgacgc tggagtcgct
     5401 ggactcccag acgatgatgc aggagagctg cctgctgcag tcgtcgtgct cgaacatgga
     5461 aaaaccatga ccgagaagga gatcgtcgac tacgtcgctt cccaggtgac tacagcaaag
     5521 aagctgcggg ggggagtcgt gttcgtcgac gaggtgccta aaggactgac aggaaagctg
     5581 gatgccagga agatccggga aatcctgatc aaggcaaaga agggcggcaa gattgccgtg
     5641 gaagtgggcg aggcagtggc caaaaggttc catctcccag gcatcagaca gggatatggc
     5701 ctgacagagt atgacatcct gactaccgtg tttcagtacg ggaaccgagc tttcacaaag
     5761 taccccgcat atgacatcct gacaactgcc tttcactacg gcaatagggt gtttgctaaa
     5821 tatcctgact tcgatattct ggcaacctcc ttcatgtatg gatctaagac ttttatcaac
     5881 catacccagt tcgatctggt gtcccacgtc ttttgctacg ggcatagagt gtttacaaaa
     5941 tatcctgagt tcgacattct cgctaccagt tttctgtacg gctcaaagac attcatcaat
     6001 cacactcagt ggccaaccct ggtgaccaca ctcacctacg gagtccagtg cttctctagg
     6061 tatcccgacg gtagaatgct catccatatt ggccgcacca agacactggt gtccactgcc
     6121 ggcgtcgaga aaatggtgtc tgtcattaag ccagaaatga agatcaaact gtgcatgagg
     6181 ggtaccgtga acggccacaa tttcgtcatt gagggagaag ggaagggtaa tccctacgag
     6241 ggtactcaga tcctggatct caacgtgacc gaaggcgcac ctctcccatt tgcctacgac
     6301 attctgacta ccgtgttcca gtatatggtc agcctgatta agcctgagat gaagatcaaa
     6361 ctgctcatgg aaggaaacgt gaatgggcac cagtttgtca tcgagggcga tggaaaaggg
     6421 catccattcg aaggaaagca gtccatggac ctggtggtca aggagggggc tcccctccct
     6481 tttgcatacg atattctgac aactgccttc cactatatgg tgggcgagga cagtgtcctc
     6541 atcaccgaaa atatgcatat gaagctgtac atggagggaa cagtgaacga ccaccatttc
     6601 aaatgtactt ccgagggtga aggcaagcca tatgagggca ctcagaccat gaagatcaaa
     6661 gtggtcgaag gagggccact ccccttcgcc tttgatattc tggctacatc cttcatgtac
     6721 atggtgtctg tcatcaagcc cgagatgaaa atgagatact atatggacgg ttctgtgaac
     6781 ggccacgagt tcaccatcga gggagaaggg accggtcgcc cttatgaggg ccatcaggaa
     6841 atgacactgc gagtgactat ggctgagggt ggccctatgc cattcgcatt tgatctggtg
     6901 tctcacgtct tctgctacat gagtgagctc atcaaagaaa acatgcacat gaaactgtac
     6961 atggagggga ccgtggacaa ccaccacttc aagtgtacaa gcgagggcga aggaaagcct
     7021 tacgagggca cacagactat gaggattaag gtggtcgaag gagggcccct gcctttcgca
     7081 tttgacatcc tcgccactag ttttctgtat atggtgtcaa agggagagga actgttcacc
     7141 ggggtggtcc caattctggt ggagctcgac ggagatgtca acgggcacaa gtttagcgtg
     7201 tccggagagg gtgaaggcga cgcaacatac ggcaagctga ctctcaaatt catctgcacc
     7261 acaggaaagc tgccagtgcc ctggcctacc ctggtcacta ccctcaccta tacactggtg
     7321 agtacagctg gagtcgagaa agcactgtca ctcggggtga ctaagctggt cgaaaggtgg
     7381 atctctgtga gtggcgtcgc tgacgatccc aacaattacc tgttctgtag agtgcggaag
     7441 aacggagtcg ctgcaccttc cgccacctct cagctcagta caagggctct ggaggggatt
     7501 tttgaagcaa cccacagact gatctacggt gccaaggacg attctggcca gagatatctg
     7561 gcctggtcag gacatagcgc ccgagtggga gcagctcgag atatggcacg agccggcgtc
     7621 agcatccctg agattatgca ggctggtggc tggacaaacg tgaatattgt catgaactac
     7681 atccggaatc tggacagcga gactggtgcc atggtccgcc tgctcgaaga cggcgatatg
     7741 tccaacctgc tcaccgtgca ccagaatctg cctgccctcc cagtggacgc tacatcagat
     7801 gaggtccgca agaacctgat ggacatgttt cgcgatcgac aggctttcag cgaacacacc
     7861 tggaagatgc tgctcagcgt gtgccgaagt tgggcagcct ggtgtaagct gaacaatagg
     7921 aaatggttcc ccgccgagcc tgaagacgtg agggattacc tgctctatct ccaggctaga
     7981 ggcctggcag tgaagacaat tcagcagcac ctgggtcagc tcaacatgct gcatcggcgc
     8041 tcaggactgc cacgaccctc agatagcaat gccgtgagcc tggtcatgcg aaggatccgg
     8101 aaagagaacg tggacgcagg agaacgagct aagcaggctc tggcatttga gagaaccgac
     8161 ttcgatcagg tgcggagcct gatggaaaac tccgaccggt gtcaggatat tcgcaatctc
     8221 gcattcctgg gcatcgccta caacactctg ctccgcatcg ccgagattgc taggatcaga
     8281 gtgaaggata tcagccgaac cgacggaggg aggatgctga tccacattgg aaggaccatg
     8341 gaggatgcaa aaaacataaa gaagggacca gcccctttct atccactgga ggatggcacc
     8401 gcaggagagc agctgcataa agcaatgaaa agatatgctc tggtccccgg aacaattgcc
     8461 ttcactgacg ctcatattga ggtcgatatt acctacgctg agtatttcga gatgtccgtc
     8521 aggctggccg aggccatgaa gcgctacggc ctcaacacca accaccggat cgtggtctgc
     8581 tctgagaata gcctgcagtt ttttatgcca gttctgggag ccctgttcat cggagttgca
     8641 gtggctcctg ctaacgacat ttataacgag cgcgagctcc tcaattccat gggaatttct
     8701 caacccactg tggtctttgt gtctaaaaag gggctgcaga agatcttgaa tgtccagaag
     8761 aagttgccaa ttattcagaa gatcattatt atggattcta agaccgatta tcagggcttc
     8821 cagagcatgt acacattcgt gactagccac cttcctcccg gatttaacga atacgatttc
     8881 gtccccgaat cctttgatcg ggacaaaact atcgcactta ttatgaacag tagcggctcc
     8941 accggcctcc ccaaaggggt ggcactgcct catagaaccg catgcgtcag gttttctcat
     9001 gcacgcgatc ctatctttgg aaatcagatt atacccgata cagctatcct ttccgtggtg
     9061 ccttttcacc atggctttgg catgttcacc actcttggct accttatttg tggctttcgg
     9121 gttgtcttga tgtatcgctt tgaagaggag ctgtttctga gaagtcttca agactataaa
     9181 attcaatccg cactcttggt cccaacactg ttctcattct tcgcaaagtc aacacttatc
     9241 gacaaatacg atctgagtaa cctgcatgag attgccagcg gcggcgcacc gctttccaag
     9301 gaagtgggag aggccgtcgc aaagagattt catagagccc actacaatat cgtcaccttc
     9361 tacccctatg atgtgcctga ttacgcttga gcggccgcta gagctcgctg atcagcctcg
     9421 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9481 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     9541 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     9601 tgggaagaca atagcaggca tgctggggat cgatgaagtt cctatacttt ctagagaata
     9661 ggaacttcgg aataggaact tctcctgcag gtcttccgct tcctcgctca ctgactcgct
     9721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    10261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    10321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10621 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10681 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10741 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10801 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10861 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10921 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10981 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11041 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    11101 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    11161 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    11221 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    11281 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    11341 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11401 tactttcacc agcgtt
//
