LOCUS       sequence-305179-        5251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMP71 expression vector containing BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Bresser K, Dijkgraaf FE, Pritchard CEJ, Huijbers IJ, Song JY, Rohr 
            JC, Scheeren FA, Schumacher TN
  TITLE     A mouse model that is immunologically tolerant to reporter and 
            modifier proteins.
  JOURNAL   Commun Biol. 2020 May 29;3(1):273. doi: 10.1038/s42003-020-0979-0.
  PUBMED    32472011
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-05-29"; volume: "3(1):273. doi"; pages: " 
            10.1038/s42003-020-0979-0"
COMMENT     Sequence Label: pMP71-BFP
FEATURES             Location/Qualifiers
     source          1..5251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1100..1109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             2009..2599
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2624..2640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2624..2640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2637..2659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2648..2664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2672..2678,2679..2696,2697..2702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2717..2738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2855..2872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3026..3614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3106..3125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3785..4576,4577..4645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4408..4427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4646..4750)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4818..4836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4874..4896)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4996..5015
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5209..5231
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5223..5240
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5224..5240
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
       61 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
      121 aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat atgggccaaa
      181 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
      241 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
      301 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
      361 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
      421 cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt
      481 ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc
      541 ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga
      601 ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc
      661 tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttggctaac
      721 tagatctgta tctggcggtc ccgcggaaga actgacgagt tcgtattccc ggccgcagcc
      781 cctgggagac gtcccagcgg cctcgggggc ccgttttgtg gcccattctg tatcagttaa
      841 cctacccgag tcggactttt tggagctccg ccactgtccg aggggtacgt ggctttgttg
      901 ggggacgaga gacagagaca cttcccgccc ccgtctgaat ttttgctttc ggttttacgc
      961 cgaaaccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
     1021 gtgtttctgt atttgtctga aaattagctc gacaaagtta agtaatagtc cctctctcca
     1081 agctcactta caggcggccg ccaccatggc caccatgtcc gaactcatca aggaaaatat
     1141 gcacatgaaa ctgtatatgg agggcaccgt cgataatcac cacttcaaat gtaccagcga
     1201 aggcgaggga aagccatacg aaggcacaca gactatgagg atcaaagtgg tcgagggagg
     1261 accactgcct ttcgcctttg acattctggc tacaagcttc ctctatggct ccaagacctt
     1321 catcaaccac actcagggaa ttcctgactt ctttaaacag tctttcccag agggcttcac
     1381 ctgggaacga gtgaccacat acgaagatgg gggtgtcctg accgccacac aggacacctc
     1441 actgcaggat gggtgcctca tctataacgt gaagattaga ggggtcaact tcaccagcaa
     1501 tggtcccgtg atgcagaaga aaacactggg gtgggaggcc ttcaccgaaa ccctgtaccc
     1561 tgccgacgga ggactggagg gtcggaatga tatggctctg aagctcgtgg ggggttctca
     1621 tctgatcgca aacatcaaga ctacctatcg cagtaagaaa ccagccaaga atctgaaaat
     1681 gcccggagtg tactatgtcg attacaggct ggagagaatc aaggaagcta acaatgagac
     1741 ctatgtggaa cagcacgaag tcgctgtggc acgctactgt gacctcccaa gcaaactcgg
     1801 acataaactg aattaaggat ccaagcttag gcctgctcgc tttcttgctg tcccatttct
     1861 attaaaggtt cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc
     1921 ttgagcatct ggattctgcc tagcgctaag cttaacacga gccatagata gaataaaaga
     1981 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     2041 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
     2101 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
     2161 agcagttcct gccccggctc agggccaaga acagttggaa cagcagaata tgggccaaac
     2221 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     2281 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     2341 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     2401 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     2461 agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc tcttgcagtt
     2521 gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactgccc
     2581 acctcggggg tctttcattc tcgagcagct tggcgtaatc atggtcatag ctgtttcctg
     2641 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2701 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2761 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2821 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2881 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4801 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4861 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4921 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4981 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5041 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     5101 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5161 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5221 gttgtaaaac gacggccagt gaattagtac t
//
