LOCUS       sequence-305183-       10908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic 
            H3F3A K27M, PDGFRA D842V, and TRP53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, 
            Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch 
            PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, 
            Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, 
            Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, 
            Breunig JJ
  TITLE     Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, 
            Stably Integrated Transgenic Elements.
  JOURNAL   Cell. 2019 Sep 19;179(1):251-267.e24. doi: 
            10.1016/j.cell.2019.08.013.
  PUBMED    31539496
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08"; journalName: "Cell"; date: "2019-09-19- 
            19"; volume: "179"; issue: "1"; pages: "251-267.e24"
COMMENT     Sequence Label: MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V ...
FEATURES             Location/Qualifiers
     source          1..10908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    32..493
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    641..775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    895..1029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1149..1283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1312..1345)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1406..1415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1838..1840,1841..2551)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1838..1840,1841..2551)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1880..1901)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2141..2160)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2488..2509
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2594..2650
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2984..2995
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5936..5992
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6365..6376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7172..7213
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7226..7814
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7279..7299)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7697..7708)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7899..7918)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7964..8019
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8018..8037)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8359..8392
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8411..8427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8411..8427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8424..8446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8435..8451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8459..8465,8466..8483,8484..8489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8504..8525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8584..8779
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8630..8765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8785..8919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8987..9004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9158..9746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9238..9257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9917..10708,10709..10777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10540..10559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10778..10882)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagtggatcc gctgtaagtc tgcagaaatt gatgatctat
       61 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
      121 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
      181 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
      241 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
      301 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
      361 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
      421 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
      481 aattccatca gaagcttgca gatctgcgac tctagaggat ctgcgactct agaggatcat
      541 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      601 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgcga
      781 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1021 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     1081 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1141 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1261 atgtatctta tcatgtctgg atccccatca agctgatccg gaacccttaa tataacttcg
     1321 tatagcatac attatacgaa gttattaggt ccctcgacct gcagcccaag ctagcttatc
     1381 gatgctagcc agctgaccgg tcgccgccac catggctcgt acaaagcaga ctgcccgcaa
     1441 atccaccggt ggtaaagcac ccaggaaaca actggctaca aaagccgctc gcatgagtgc
     1501 gccctctact ggaggggtga agaaacctca tcgttacagg cctggtactg tggccctccg
     1561 tgaaatcaga cgctatcaga agtccactga acttctgatc cgcaagctcc cctttcagcg
     1621 tctggtgcga gaaattgctc aggacttcaa aacagatctg cgcttccaga gtgcagctat
     1681 tggtgctttg caggaggcaa gtgaggccta tctggttggc ctttttgaag ataccaatct
     1741 gtgtgctatc catgccaaac gtgtaacaat tatgccaaaa gatatccagc tagcacgccg
     1801 catacgcgga gaacgtgctt cgagaccggt agctactgtg agcaagggcg aggagctgtt
     1861 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1921 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1981 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2041 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2101 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2161 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2221 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2281 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2341 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2401 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2461 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2521 gatcactctc ggcatggacg agctgtacaa gggcggcgat acctatcggt atattggatc
     2581 cggaggatcc ggagccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa
     2641 ccccggtcct atggggacct cccaccaggt ctttctggtc ctcagctgtc tcctcacagg
     2701 gccgggcctc atctcctgcc agctcttatt accctctatc ctcccaaacg agaatgagaa
     2761 gattgtgcag ctgaattcgt ccttttctct gagatgcgtt ggggagagtg aagtgagctg
     2821 gcagcacccc atgtctgaag aagacgaccc caacgtggaa atcagaagtg aggagaacaa
     2881 cagtggcctc tttgtcacag tgctggaagt ggttaacgcc tccgcagccc acaccggatg
     2941 gtacacttgc tactacaacc acactcagac ggatgagagt gagatcgaag gcaggcacat
     3001 ttacatctat gtaccagacc cagacatggc ctttgtgcct ctcgggatga ccgattcctt
     3061 agtcatcgtg gaagaggatg actctgccat cataccttgc cgcaccacag atccggagac
     3121 tcaagtaacc ttgcacaata acgggaggct ggtgcctgcc tcctatgaca gcaggcaggg
     3181 cttcaacgga accttcagcg tggggcctta catctgtgag gccaccgtca aagggaggac
     3241 gttcaagacc agcgagttta atgtttatgc cttgaaagca acgtcagaac tgaatctgga
     3301 gatggacgca cgccagactg tgtataaggc aggagaaacg atcgtggtga cctgtgccgt
     3361 ctttaacaac gaggtcgttg acctgcagtg gacttaccct ggagaagtga gaaacaaagg
     3421 tatcaccatg ctggaggaga tcaaactccc atccatcaaa ctggtgtaca ctttgaccgt
     3481 ccccaaggcc acggtgaagg acagtggaga gtatgaatgt gctgcccgtc aggccactaa
     3541 agaggtcaag gaaatgaaga gagtcaccat ttctgtccac gagaaaggct tcgtcgagat
     3601 tgagcccacc tttggccagc tggaagctgt caacttgcac gaagtcagag agttcgtggt
     3661 ggaggtgcag gcctacccga cgcccaggat atcgtggctg aaggacaact tgactctgat
     3721 agagaatctc accgagatca ccactgatgt acagaagagt caggaaacaa ggtatcagag
     3781 caaattaaag ctgatccggg ctaaggaaga agacagtggc cattacacca ttatagttca
     3841 aaatgaggat gacgtgaaga gttacacgtt tgagctgtca accctagttc ctgcatccat
     3901 tttggatctc gtagatgacc accatggctc tggcggggga cagactgtga ggtgtacagc
     3961 cgaaggcacc cctcttccag aaatcgactg gatgatctgc aagcatatta agaaatgtaa
     4021 taatgacact tcgtggacag ttttggccag caatgtctca aatattatca cagagctccc
     4081 tcgccgtggc aggagtaccg tggagggacg agtgtccttc gccaaagtgg aagagaccat
     4141 cgcagttcga tgcctggcaa agaacaacct cagcgttgtg gcccgtgagc tgaagctggt
     4201 ggctcccact ctgcgatctg aactcacagt ggcggccgca gtgttggtgc tgttggtgat
     4261 tgtcattgtc tctctcatcg tcctggtggt catttggaag cagaaaccac ggtatgaaat
     4321 tcgctggagg gttatcgagt caatcagccc cgatggacat gagtatatct atgtggaccc
     4381 catgcagttg ccttacgact ccagatggga attcccgaga gatggcctcg tgcttggtcg
     4441 gattttggga tccggtgcat ttgggaaagt ggtcgaaggt acagcttatg gattaagccg
     4501 gtcccaacct gtaatgaagg tggctgtgaa gatgctcaaa cccacagcca ggtctagtga
     4561 aaagcaggct ctcatgtctg agctgaagat aatgactcac ctggggccac atttgaacat
     4621 tgtgaacttg ctgggagcct gcaccaagtc aggtcccatt tacatcatca cagaatactg
     4681 cttctatggg gatttggtca actacttgca taagaacaga gatagcttca tgagccaaca
     4741 cccagagaag ccgaagaaag acctggacat ctttggattg aatcctgcag acgagagcac
     4801 aagaagttat gtgattttgt cttttgaaaa taacggcgac tacatggaca tgaagcaagc
     4861 tgataccaca cagtatgtcc ccatgcttga aaggaaagag gtttctaaat actctgacat
     4921 ccagagatcg ctgtacgatc ggccagcctc ctacaagaag aaatccatgc tagactcaga
     4981 agtcaaaaac ctcctttcgg acgatgactc cgagggtctg actttgctgg atctattgag
     5041 cttcacctat caagttgctc gaggaatgga atttttggct tcgaaaaatt gtgtccaccg
     5101 ggacctggct gcccgcaacg tcctcctggc gcaaggaaaa attgtgaaga tctgtgattt
     5161 tggcctggcc agagtcatca tgcacgattc caactacgtg tcaaaaggca gtactttcct
     5221 gcctgtgaag tggatggcac ccgagagcat cttcgacaac ctctacacca cgctgagtga
     5281 cgtctggtcc tatggcattc tgctctggga aatcttttcc cttggtggca caccctatcc
     5341 tggcatgatg gtcgattcta ctttctacaa taagatcaag agtggatacc ggatggccaa
     5401 acctgaccat gccaccagtg aagtctatga gatcatggtg cagtgctgga acagtgagcc
     5461 cgagaagaga ccctccttct accacctcag cgagatagtg gagaacctgt tgccgggaca
     5521 atacaagaag agttatgaaa agattcacct ggacttccta aagagtgacc atccggccgt
     5581 ggcacgcatg cgggtggact ctgataatgc gtacatcggt gtcacttaca aaaatgaaga
     5641 ggataagctg aaggactggg aaggtggcct ggacgaacag agactgagcg ctgacagtgg
     5701 ctacatcatc cccctgccag acattgaccc tgttccagag gaggaagacc tgggcaagag
     5761 gaacagacac agctcacaga cttcggaaga gagtgccatc gagacaggtt ccagtagttc
     5821 caccttcatc aagagagagg acgagaccat cgaggacatc gacatgatgg atgacattgg
     5881 tatagattcc tcggacctgg tggaggacag cttcctgggc agtggaggga gtggagcaac
     5941 caatttttca ctcctgaagc aagctgggga tgtagaggag aatcctgggc ctatgactgc
     6001 catggaggag tcacagtcgg atatcagcct cgagctccct ctgagccagg agacattttc
     6061 aggcttatgg aaactacttc ctccagaaga tatcctgcca tcacctcact gcatggacga
     6121 tctgttgctg ccccaggatg ttgaggagtt ttttgaaggc ccaagtgaag ccctccgagt
     6181 gtcaggagct cctgcagcac aggaccctgt caccgagacc cctgggccag tggcccctgc
     6241 cccagccact ccatggcccc tgtcatcttt tgtcccttct caaaaaactt accagggcaa
     6301 ctatggcttc cacctgggct tcctgcagtc tgggacagcc aagtctgtta tgtgcacgta
     6361 ctctcctccc ctcaataagc tattctgcca gctggcgaag acgtgccctg tgcagttgtg
     6421 ggtcagcgcc acacctccag ctgggagccg tgtccgcgcc atggccatct acaagaagtc
     6481 acagcacatg acggaggtcg tgagacgctg cccccaccat gagcgctgct ccgatggtga
     6541 tggcctggct cctccccagc atcttatccg ggtggaagga aatttgtatc ccgagtatct
     6601 ggaagacagg cagacttttc gccacagcgt ggtggtacct tatgagccac ccgaggccgg
     6661 ctctgagtat accaccatcc actacaagta catgtgtaat agctcctgca tggggggcat
     6721 gaaccgccga cctatcctta ccatcatcac actggaagac tccagtggga accttctggg
     6781 acgggacagc tttgaggttc atgtttgtgc ctgccctggg agagaccgcc gtacagaaga
     6841 agaaaatttc cgcaaaaagg aagtcctttg ccctgaactg cccccaggga gcgcaaagag
     6901 agcgctgccc acctgcacaa gcgcctctcc cccgcaaaag aaaaaaccac ttgatggaga
     6961 gtatttcacc ctcaagatcc gcgggcgtaa acgcttcgag atgttccggg agctgaatga
     7021 ggccttagag ttaaaggatg cccatgctac agaggagtct ggagacagca gggctcactc
     7081 cagctacctg aagaccaaga agggccagtc tacttcccgc cataaaaaaa caatggtcaa
     7141 gaaagtgggg cctgactcag acggtggctc aggtaagcct atccctaacc ctctcctcgg
     7201 tctcgattct acgtaagcgg ccgctaatca acctctggat tacaaaattt gtgaaagatt
     7261 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7321 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7381 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7441 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7501 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7561 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7621 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7681 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7741 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7801 ggccgcctcc ccgcaggccg cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     7861 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     7921 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     7981 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     8041 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     8101 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     8161 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     8221 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     8281 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     8341 agatccctcg acctgcagga agttcctatt ctctagaaag tataggaact tcaagcttgg
     8401 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8461 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8521 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     8581 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8641 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8701 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8761 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     8821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8881 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     8941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
