LOCUS       sequence-305190-        8003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3-color stripe (inverted reporters).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2251 to 2285)
  AUTHORS   Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
  TITLE     Multistable and dynamic CRISPRi-based synthetic circuits.
  JOURNAL   Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1.
  PUBMED    32488086
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-2"; volume: "11(1):2746. doi"; pages: " 
            10.1038/s41467-020-16574-1"
COMMENT     Sequence Label: pJ2042.2_invRep
FEATURES             Location/Qualifiers
     source          1..8003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..349
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     272..291
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    410..429
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    532..551
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    612..631
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    738..757
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    824..843
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             848..870
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             878..1531
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     promoter        2251..2285
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     protein_bind    2435..2454
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             2459..2481
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        3730..3764
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        3730..3764
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        3730..3764
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     protein_bind    3908..3927
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    4034..4053
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    4120..4139
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             4144..4166
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4174..4887
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      5237..5264
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5452..6267)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5452..6267)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6175..6194
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(6268..6359)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6377..7012)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(6522..6541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7163..8003,1..38))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
       61 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
      121 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
      181 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
      241 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
      301 gcggttccta cctgacgctt tttatcgcaa ctctctactg tttctccata ccgaattcat
      361 aggatagatt ctggaaactt taccgtccga gctccagcct gcggtccggg ttcactgccg
      421 tataggcaga agctagactc tagtggtttc agagctatgc tggaaacagc atagcaagtt
      481 gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cgttcactgc
      541 cgtataggca gtcgctggga cgcccgggga ctacacttac gaaactattg attgctcagc
      601 ctgcggtccg ggttcactgc cgtataggca gatcagtgtg tactaagtac tgtttcagag
      661 ctatgctgga aacagcatag caagttgaaa taaggctagt ccgttatcaa cttgaaaaag
      721 tggcaccgag tcggtgcgtt cactgccgta taggcagtcg ctgggacgcc cgctcgagca
      781 ataaacagtt gatagggctt ctccgttaca gcctgcggtc cgggttcact gccgtatagg
      841 cagtaatttt gtttaacttt aagaaggaga tatacatatg gtttcggtta tcaaaccaga
      901 gatgaaaatg cgttactata tggatggttc agtaaatggt cacgaattta ctattgaggg
      961 cgagggtacg ggacgcccat acgaggggca ccaggaaatg actttacgcg tcacaatggc
     1021 tgaaggcggg cctatgccgt ttgcgttcga tcttgttagt catgtctttt gttacggtca
     1081 ccgtgtattt actaaatacc ccgaggaaat tccagactat ttcaaacaag ccttcccgga
     1141 aggtttgtct tgggagcgca gtttagagtt tgaagacggt ggctcggcca gcgtgtcagc
     1201 tcatattagt cttcgcggca atacatttta tcacaagtca aagttcaccg gcgtgaactt
     1261 ccccgcagac ggcccaatca tgcagaatca aagtgttgat tgggaaccgt ccacagagaa
     1321 gattacagct tccgatggag tcttaaaggg cgatgtaacc atgtacttaa aattagaagg
     1381 gggagggaac cataaatgtc agatgaagac tacctataag gccgcaaaag agattcttga
     1441 aatgcccgga gaccactaca ttgggcatcg tttggtccgt aagacagaag gaaatattac
     1501 tgaacaggtc gaagacgctg tggcacacag catgtcccgc cgtaatactg acgccatcac
     1561 aatccacagc atcctggatt ggattgaaga caacttggag tcgccgttga gtttagaaaa
     1621 agttagtgaa cgtagtggtt actcaaagtg gcaccttcag cgcatgttta agaaggaaac
     1681 gggtcattca ttgggtcaat atattcgttc tcgcaagatg actgaaattg cccagaaatt
     1741 gaaagagtct aatgaaccta ttttgtacct ggcggagcgt tacggctttg aaagtcagca
     1801 aacccttaca cgtaccttca agaattactt tgacgttcca ccacacaaat atcgtatgac
     1861 caacatgcag ggtgagtcac gttttttgca tccgttgaat cattacaatt cctaataatc
     1921 gctgggacgc ccgccatggt tcagccaaaa aacttaagac cgccggtctt gtccactacc
     1981 ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc tcttctcaat tcttctgacc
     2041 tgtaacgaat aatagatagt aaagtagtct ccgattgagt tttctctgcc gagtcccacc
     2101 cagttctgtg atttcagtaa gttggtaatt gatacactgt tgcgagaact gctgcctggt
     2161 agtagatagg ttgttattga gtaagaaggt aaagtgaacg aaatccctga aactgagact
     2221 gtagaaaata agcttcagcc tgcggtccgg ttgacggcta gctcagtcct aggtacagtg
     2281 ctagctcgct gggacgcccg agatagccgt tacacaggtg acacttattt cagcctgcgg
     2341 tccggccaca ctagagtcta gcttgagatc gctgggacgc ccgggatcca agagatttct
     2401 acacgattga gcactgtctc agcctgcggt ccgggttcac tgccgtatag gcagtaattt
     2461 tgtttaactt taagaaggag atatacatat gaatcagtca ttcatctcgg acatcttata
     2521 tgccgacatc gaatcgaagg ctaaggaact tacagtcaat tccaacaata ctgtccagcc
     2581 ggtcgcgctt atgcgcttag gagttttcgt tcccaaacct tccaagagca aaggagaaag
     2641 taaggaaatt gacgccacca aagccttctc tcaactggag attgctaaag cagagggcat
     2701 ggttagtaaa ggagaagaaa ataacatggc actgattaag gagaacatgc acatgaagct
     2761 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
     2821 gccctacgag ggcacccaga ccatgagaat caaggccgtc gagggcggcc ctctcccctt
     2881 cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac
     2941 ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt
     3001 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     3061 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
     3121 gcagaagaaa acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg
     3181 cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
     3241 cttgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
     3301 ctatgtggac agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca
     3361 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acagagtcta
     3421 ataatcgctg ggacgcccgc ctgcaggctc ggtaccaaat tccagaaaag aggcctcccg
     3481 aaaggggggc cttttttcgt tttggtccta atagataaag gataggtctg gtagtgttgt
     3541 tcgttctcgc aggtaaatca ataatactca gcagttccgt agacttttca gtgggacagg
     3601 gtagcgataa cagatagatt gtaataagac acagtaggtg ctcgtagttg cgtgaagaga
     3661 accgctcagg aaatccagtc agaagtattg gtaatcgttg aaaactcagt cgaccagcct
     3721 gcggtccggt ttacggctag ctcagtccta ggtattatgc tagctcgctg ggacgcccga
     3781 gtgacgactg cgaagtaacc tctatttatc agcctgcggt ccggccaagt acttagtaca
     3841 cactgattcg ctgggacgcc cggtagtgct tatcagaccc aatactgttg aacagcctgc
     3901 ggtccgggtt cactgccgta taggcagtct caagctagac tctagtggtt tcagagctat
     3961 gctggaaaca gcatagcaag ttgaaataag gctagtccgt tatcaacttg aaaaagtggc
     4021 accgagtcgg tgcgttcact gccgtatagg cagtcgctgg gacgcccgga cgtcctatta
     4081 cactcgtcgt tggaaactga agatcagcct gcggtccggg ttcactgccg tataggcagt
     4141 aattttgttt aactttaaga aggagatata catatgcgta aaggcgaaga actgtttacc
     4201 ggtgtggttc cgattctggt ggaactggac ggcgatgtta atggtcataa attcagtgtt
     4261 cgcggcgaag gtgaaggcga tgcgacgaac ggcaaactga ccctgaaatt tatctgcacc
     4321 acgggtaaac tgccggtccc gtggccgacg ctggtgacca cgctgaccta tggcgttcaa
     4381 tgttttgcgc gttacccgga tcacatgaaa cagcacgact ttttcaaatc ggccatgccg
     4441 gaaggctatg tgcaggaacg tacgattagc tttaaagacg atggtacgta taaaacccgc
     4501 gcggaagtga aattcgaagg cgataccctg gttaaccgta tcgaactgaa aggtatcgat
     4561 ttcaaagaag acggcaatat tctgggtcat aaactggaat ataacttcaa ttcccacaac
     4621 gtgtacatca ccgcggataa acagaaaaac ggcattaaag ccaatttcaa aatccgccat
     4681 aatgtggaag atggtagcgt tcagctggcc gaccactatc agcaaaacac gccgattggt
     4741 gatggcccgg tcctgctgcc ggacaatcac tacctgagta cccagtccgt gctgtcaaaa
     4801 gatccgaacg aaaaacgtga ccacatggtc ctgctggaat ttgtgacggc tgcgggtatc
     4861 acccacggca tggacgaact gtataaaatg tcccgccgta atactgacgc catcacaatc
     4921 cacagcatcc tggattggat tgaagactaa taatcgctgg gacgcccggc ggccgcggaa
     4981 acacagaaaa aagcccgcac ctgacagtgc gggctttttt tttcgaccaa aggtagcgaa
     5041 cgacgagtca ctgttgagga taaatacttt ctctactagg cgcctgttac acaggtcctc
     5101 agcggcgcgc ctttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     5161 ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     5221 aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tcagatctac
     5281 cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgacaa
     5341 gctgacgacc gggtctccgc aagtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     5461 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     5521 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     5581 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     5641 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     5701 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     5761 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     5821 gagacgaaat acgcggtcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     5881 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     5941 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     6001 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     6061 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     6121 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     6181 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca
     6241 agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta
     6301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6361 aggcatgcta gcgcagaaac gtcctagaag atgccaggag gatacttagc agagagacaa
     6421 taaggccgga gcgaagccgt ttttccatag gctccgcccc cctgacgaac atcacgaaat
     6481 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6541 ccctgatggc tccctcttgc gctctcctgt tcccgtcctg cggcgtccgt gttgtggtgg
     6601 aggctttacc caaatcacca cgtcccgttc cgtgtagaca gttcgctcca agctgggctg
     6661 tgtgcaagaa ccccccgttc agcccgactg ctgcgcctta tccggtaact atcatcttga
     6721 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattggta actgagaatt
     6781 agtggattta gatatcgaga gtcttgaagt ggtggcctaa cagaggctac actgaaagga
     6841 cagtatttgg tatctgcgct ccactaaagc cagttaccag gttaagcagt tccccaactg
     6901 acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacaga gcaggagatt
     6961 acgacgatcg taaaaggatc tcaagaagat cctttacgga ttcccgacac catcactcta
     7021 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
     7081 gttgtaattc tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgctc
     7141 ctagggtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
     7201 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     7261 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     7321 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     7381 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     7441 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     7501 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     7561 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     7621 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     7681 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     7741 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     7801 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     7861 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     7921 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     7981 ccaccagatg ggcattaaac gag
//
