LOCUS       sequence-305218-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3507
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             273..284
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtaaga ggatctcgcc ctcggcagat
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggcaatgaa agtccatcag attcctcagt aaagctgaag
      241 tcacacacta gagacaggtg gtctgaagga taattgaagg aaggtaacct gttgggtcca
      301 atctgttctt cagtgagcag atcgagagct gaccttacat ttagagcatg tttagaatac
      361 cagatgtaat ccagggtgtg cctgcactcc cctgaggtcc ggatcttcca ggtagtgtat
      421 gggggttctg actgcccatc agcactcagc agcttgtagg cgctgttcag gttgaggctg
      481 gaggaagcaa agtgtttgta gacctcttct gttggctctg cattgaagtc cccacacaca
      541 ataaggggaa tcttggctcc ttgggtgatg ttttgcaggt tctgaaggag gtcacagcct
      601 tgagctgatc gaaaccgctc ccagccagtg cgtgctttta gatgggtaac agcgatgcag
      661 aactgtcggc ctgactcctt gcactc
//
