LOCUS       sequence-305232-        8003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3-color stripe (different sgRNAs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3117 to 3151)
  AUTHORS   Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
  TITLE     Multistable and dynamic CRISPRi-based synthetic circuits.
  JOURNAL   Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1.
  PUBMED    32488086
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-06-2"; volume: "11(1):2746. doi"; pages: " 
            10.1038/s41467-020-16574-1"
COMMENT     Sequence Label: pJ2048.2
FEATURES             Location/Qualifiers
     source          1..8003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(26..904)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        931..1215
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1138..1157
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     protein_bind    1276..1295
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    1402..1421
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    1482..1501
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    1604..1623
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    1690..1709
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             1714..1736
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1744..2397
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     promoter        3117..3151
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     protein_bind    3301..3320
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             3325..3347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3355..4068
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        4440..4474
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     promoter        4440..4474
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     promoter        4440..4474
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     protein_bind    4618..4637
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    4744..4763
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     protein_bind    4830..4849
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             4854..4876
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      6103..6130
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(6318..7133)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6318..7133)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7041..7060
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7134..7225)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7243..7878)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(7388..7407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcctagggt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
       61 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      121 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      181 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      241 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      301 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      361 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      421 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      481 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      541 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      601 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      661 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      721 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      781 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      841 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
      901 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
      961 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1021 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1081 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1141 ccatagcatt tttatccata agattagcgg ttcctacctg acgcttttta tcgcaactct
     1201 ctactgtttc tccataccga attcatagga tagattctgg aaactttacc gtccgagctc
     1261 cagcctgcgg tccgggttca ctgccgtata ggcagtgact gagctagtgt actctgtttc
     1321 agagctatgc tggaaacagc atagcaagtt gaaataaggc tagtccgtta tcaacttgaa
     1381 aaagtggcac cgagtcggtg cgttcactgc cgtataggca gtcgctggga cgcccgggga
     1441 ctacacttac gaaactattg attgctcagc ctgcggtccg ggttcactgc cgtataggca
     1501 gcatcttaga gtatgtagtt tcagagctat gctggaaaca gcatagcaag ttgaaataag
     1561 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcgttcact gccgtatagg
     1621 cagtcgctgg gacgcccgct cgagcaataa acagttgata gggcttctcc gttacagcct
     1681 gcggtccggg ttcactgccg tataggcagt aattttgttt aactttaaga aggagatata
     1741 catatggttt cggttatcaa accagagatg aaaatgcgtt actatatgga tggttcagta
     1801 aatggtcacg aatttactat tgagggcgag ggtacgggac gcccatacga ggggcaccag
     1861 gaaatgactt tacgcgtcac aatggctgaa ggcgggccta tgccgtttgc gttcgatctt
     1921 gttagtcatg tcttttgtta cggtcaccgt gtatttacta aataccccga ggaaattcca
     1981 gactatttca aacaagcctt cccggaaggt ttgtcttggg agcgcagttt agagtttgaa
     2041 gacggtggct cggccagcgt gtcagctcat attagtcttc gcggcaatac attttatcac
     2101 aagtcaaagt tcaccggcgt gaacttcccc gcagacggcc caatcatgca gaatcaaagt
     2161 gttgattggg aaccgtccac agagaagatt acagcttccg atggagtctt aaagggcgat
     2221 gtaaccatgt acttaaaatt agaaggggga gggaaccata aatgtcagat gaagactacc
     2281 tataaggccg caaaagagat tcttgaaatg cccggagacc actacattgg gcatcgtttg
     2341 gtccgtaaga cagaaggaaa tattactgaa caggtcgaag acgctgtggc acacagcatg
     2401 tcccgccgta atactgacgc catcacaatc cacagcatcc tggattggat tgaagacaac
     2461 ttggagtcgc cgttgagttt agaaaaagtt agtgaacgta gtggttactc aaagtggcac
     2521 cttcagcgca tgtttaagaa ggaaacgggt cattcattgg gtcaatatat tcgttctcgc
     2581 aagatgactg aaattgccca gaaattgaaa gagtctaatg aacctatttt gtacctggcg
     2641 gagcgttacg gctttgaaag tcagcaaacc cttacacgta ccttcaagaa ttactttgac
     2701 gttccaccac acaaatatcg tatgaccaac atgcagggtg agtcacgttt tttgcatccg
     2761 ttgaatcatt acaattccta ataatcgctg ggacgcccgc catggttcag ccaaaaaact
     2821 taagaccgcc ggtcttgtcc actaccttgc agtaatgcgg tggacaggat cggcggtttt
     2881 cttttctctt ctcaattctt ctgacctgta acgaataata gatagtaaag tagtctccga
     2941 ttgagttttc tctgccgagt cccacccagt tctgtgattt cagtaagttg gtaattgata
     3001 cactgttgcg agaactgctg cctggtagta gataggttgt tattgagtaa gaaggtaaag
     3061 tgaacgaaat ccctgaaact gagactgtag aaaataagct tcagcctgcg gtccggttga
     3121 cggctagctc agtcctaggt acagtgctag ctcgctggga cgcccgagat agccgttaca
     3181 caggtgacac ttatttcagc ctgcggtccg gccatacata ctctaagatg tgtctcgctg
     3241 ggacgcccgg gatccaagag atttctacac gattgagcac tgtctcagcc tgcggtccgg
     3301 gttcactgcc gtataggcag taattttgtt taactttaag aaggagatat acatatgcgt
     3361 aaaggcgaag aactgtttac cggtgtggtt ccgattctgg tggaactgga cggcgatgtt
     3421 aatggtcata aattcagtgt tcgcggcgaa ggtgaaggcg atgcgacgaa cggcaaactg
     3481 accctgaaat ttatctgcac cacgggtaaa ctgccggtcc cgtggccgac gctggtgacc
     3541 acgctgacct atggcgttca atgttttgcg cgttacccgg atcacatgaa acagcacgac
     3601 tttttcaaat cggccatgcc ggaaggctat gtgcaggaac gtacgattag ctttaaagac
     3661 gatggtacgt ataaaacccg cgcggaagtg aaattcgaag gcgataccct ggttaaccgt
     3721 atcgaactga aaggtatcga tttcaaagaa gacggcaata ttctgggtca taaactggaa
     3781 tataacttca attcccacaa cgtgtacatc accgcggata aacagaaaaa cggcattaaa
     3841 gccaatttca aaatccgcca taatgtggaa gatggtagcg ttcagctggc cgaccactat
     3901 cagcaaaaca cgccgattgg tgatggcccg gtcctgctgc cggacaatca ctacctgagt
     3961 acccagtccg tgctgtcaaa agatccgaac gaaaaacgtg accacatggt cctgctggaa
     4021 tttgtgacgg ctgcgggtat cacccacggc atggacgaac tgtataaaat gtcccgccgt
     4081 aatactgacg ccatcacaat ccacagcatc ctggattgga ttgaagacta ataatcgctg
     4141 ggacgcccgc ctgcaggctc ggtaccaaat tccagaaaag aggcctcccg aaaggggggc
     4201 cttttttcgt tttggtccta atagataaag gataggtctg gtagtgttgt tcgttctcgc
     4261 aggtaaatca ataatactca gcagttccgt agacttttca gtgggacagg gtagcgataa
     4321 cagatagatt gtaataagac acagtaggtg ctcgtagttg cgtgaagaga accgctcagg
     4381 aaatccagtc agaagtattg gtaatcgttg aaaactcagt cgaccagcct gcggtccggt
     4441 ttacggctag ctcagtccta ggtattatgc tagctcgctg ggacgcccga gtgacgactg
     4501 cgaagtaacc tctatttatc agcctgcggt ccggccaaga gtacactagc tcagtcatcg
     4561 ctgggacgcc cggtagtgct tatcagaccc aatactgttg aacagcctgc ggtccgggtt
     4621 cactgccgta taggcaggac acatcttaga gtatgtagtt tcagagctat gctggaaaca
     4681 gcatagcaag ttgaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     4741 tgcgttcact gccgtatagg cagtcgctgg gacgcccgga cgtcctatta cactcgtcgt
     4801 tggaaactga agatcagcct gcggtccggg ttcactgccg tataggcagt aattttgttt
     4861 aactttaaga aggagatata catatgaatc agtcattcat ctcggacatc ttatatgccg
     4921 acatcgaatc gaaggctaag gaacttacag tcaattccaa caatactgtc cagccggtcg
     4981 cgcttatgcg cttaggagtt ttcgttccca aaccttccaa gagcaaagga gaaagtaagg
     5041 aaattgacgc caccaaagcc ttctctcaac tggagattgc taaagcagag ggcatggtta
     5101 gtaaaggaga agaaaataac atggcactga ttaaggagaa catgcacatg aagctgtaca
     5161 tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct
     5221 acgagggcac ccagaccatg agaatcaagg ccgtcgaggg cggccctctc cccttcgcct
     5281 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     5341 gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca
     5401 catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     5461 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     5521 agaaaacact cggctgggag gcctccaccg agaccctgta ccccgctgac ggcggcctgg
     5581 aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     5641 agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     5701 tggacagaag actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg
     5761 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga gtctaataat
     5821 cgctgggacg cccggcggcc gcggaaacac agaaaaaagc ccgcacctga cagtgcgggc
     5881 tttttttttc gaccaaaggt agcgaacgac gagtcactgt tgaggataaa tactttctct
     5941 actaggcgcc tgttacacag gtcctcagcg gcgcgccttt gtcggtgaac gctctcctga
     6001 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc
     6061 gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg
     6121 atggcctttt tgcgtttcag atctaccggt aaaccagcaa tagacataag cggctattta
     6181 acgaccctgc cctgaaccga cgacaagctg acgaccgggt ctccgcaagt ggcacttttc
     6241 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6301 cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     6361 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     6421 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     6481 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     6541 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     6601 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     6661 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc ggtcgctgtt aaaaggacaa
     6721 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     6781 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     6841 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     6901 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     6961 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     7021 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     7081 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc
     7141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7201 gaatgtattt agaaaaataa acaaataggc atgctagcgc agaaacgtcc tagaagatgc
     7261 caggaggata cttagcagag agacaataag gccggagcga agccgttttt ccataggctc
     7321 cgcccccctg acgaacatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     7381 ggactataaa gataccaggc gtttccccct gatggctccc tcttgcgctc tcctgttccc
     7441 gtcctgcggc gtccgtgttg tggtggaggc tttacccaaa tcaccacgtc ccgttccgtg
     7501 tagacagttc gctccaagct gggctgtgtg caagaacccc ccgttcagcc cgactgctgc
     7561 gccttatccg gtaactatca tcttgagtcc aacccggaaa gacacgacaa aacgccactg
     7621 gcagcagcca ttggtaactg agaattagtg gatttagata tcgagagtct tgaagtggtg
     7681 gcctaacaga ggctacactg aaaggacagt atttggtatc tgcgctccac taaagccagt
     7741 taccaggtta agcagttccc caactgactt aaccttcgat caaaccgcct ccccaggcgg
     7801 ttttttcgtt tacagagcag gagattacga cgatcgtaaa aggatctcaa gaagatcctt
     7861 tacggattcc cgacaccatc actctagatt tcagtgcaat ttatctcttc aaatgtagca
     7921 cctgaagtca gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc
     7981 accggaagga gctgactggg ttg
//
