LOCUS       sequence-30526-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Ho L, Ronan JL, Wu J, Staahl BT, Chen L, Kuo A, Lessard J, 
            Nesvizhskii AI, Ranish J, Crabtree GR
  TITLE     An embryonic stem cell chromatin remodeling complex, esBAF, is 
            essential for embryonic stem cell self-renewal and pluripotency.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6.
  PUBMED    19279220
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Mar 31. 106(13):5181-6."
COMMENT     Sequence Label: pRRL CAGpNFlag BAF155 ires GFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(369..392)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(483..505)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gctgccccaa gaatgtgaca caaggtgcct ccagctttga aatccatgaa acattttgca
       61 ggtagtttgg tgaaagctgg gttggtgaca tgcttcccaa aggcatcttc ttggaactgt
      121 agaagctgca ccaccagtcc agctagtgtt ttattggtag gagcatctgc atgaacatac
      181 ttcttgtagt gcttgcccag ccagactcgc accgaatcta gctgggacac cgtgtccggg
      241 ctctcccaaa acttgctggc cgggcccccg tccttcctcc ggtacacggc cagcccggag
      301 gccgccgccg caccccctgc acccacggcg cctgctgctg ctcccggacc gccacccgct
      361 gtcgcggcct tatcgtcgtc atccttgtaa tccatggtgg accggtggat ccccctgggg
      421 agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct cctagagccg
      481 ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc
      541 ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg cgacgcacga
      601 aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta
      661 gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga caaaggaagt
      721 ccctgcgcgc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga
      781 cagccgccgg cgctccgggg gccgccgcgc ccct
//
