LOCUS       sequence-30528-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Ho L, Ronan JL, Wu J, Staahl BT, Chen L, Kuo A, Lessard J, 
            Nesvizhskii AI, Ranish J, Crabtree GR
  TITLE     An embryonic stem cell chromatin remodeling complex, esBAF, is 
            essential for embryonic stem cell self-renewal and pluripotency.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 31. 106(13):5181-6.
  PUBMED    19279220
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Mar 31. 106(13):5181-6."
COMMENT     Sequence Label: pRRL CAG NFlag BAF170 ires GFP
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(409..432)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(526..548)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ggaggcatta ttctgtacca aggacttctc aatggtcatg aacatttcca cattgcggtc
       61 catgcgtgat ggattctgga aatcgtaacg ccgccatccc tggtcactct tgaatttgta
      121 ggcagctgca agaatatggc agagggatcc tcctgctttg aaatctagga aacatttgat
      181 cggcagttta gtaagcggtg cgttgctgac atgtttgcca aaaacctctt cctgaaactg
      241 gagcaactgc accaccaggc tggacagaga cttgttggtt ggcggttctg cttgtatgta
      301 cttcttgtag ttcttgccga gccagagccg cacgttgtcg aactgggtca cggtgtccgc
      361 ggcctcgtag tacttcacgt tggggccgcc gtccttcttc cgcaccgcct tatcgtcgtc
      421 atccttgtaa tccatggtgg accggtggat ccccctgggg agagaggtcg gtgattcggt
      481 caacgaggga gccgactgcc gacgtgcgct ccggctagag ccgccggtca cacgccagaa
      541 gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg
      601 aggctggaga gggagaaggg gacggcggcg cggcgacgca cgaaggccct ccccgcccat
      661 ttccttcctg ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg
      721 cctcccagat ttcggctccg cacagatttg ggacaaagga agtccctgcg ccctctcgca
      781 cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cgncgctccg
      841 ggggccgccg cgcccctncc ccgag
//
