LOCUS       sequence-305305-        7546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 
            (Prx1) oligomerization (for stable cell line generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Pastor-Flores D, Talwar D, Pedre B, Dick TP
  TITLE     Real-time monitoring of peroxiredoxin oligomerization dynamics in 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 
            10.1073/pnas.1915275117.
  PUBMED    32601209
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 
            10.1073/pnas.1915275117."
COMMENT     Sequence Label: pLPCX mCER-Prx1
FEATURES             Location/Qualifiers
     source          1..7546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             65..658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(761..783)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    911..944
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1004..1037
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1057..1076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1157..1174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1328..1916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1408..1427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2087..2878,2879..2947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2710..2729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2948..3052)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3120..3138
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             3740..3915
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    3978..4177
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4378..4794
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4724..4746
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4762..4778
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4892..5491
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4892..4911)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5388..5408
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        5666..5969
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5970..6173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6124..6144
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(6224..6226,6227..6229,6230..6940)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6269..6290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6530..6549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6877..6898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcggccgcct cggccaaaca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
       61 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
      121 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
      181 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      241 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
      301 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
      361 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      421 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
      481 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
      541 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
      601 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
      661 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
      721 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      841 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
      901 gagtgtactg ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
      961 tttacgtcca gccaagctag ggccgcgatc cggaaccctt aatataactt cgtataatgt
     1021 atgctatacg aagttatcag tactggctta actatgcggc atcagagcag attgtactga
     1081 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1141 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1201 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1261 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1321 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1381 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1501 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1561 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1741 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2401 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2701 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3061 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3121 ataggcgtat cacgaggccc tttcgtcttc aagaattgct agcaattgct agcaattgct
     3181 agcaattcat accagatcac cgaaaactgt cctccaaatg tgtccccctc acactcccaa
     3241 attcgcgggc ttctgcctct tagaccactc taccctattc cccacactca ccggagccaa
     3301 agccgcggcc cttccgtttc tttgcttttg aaagacccca cccgtaggtg gcaagctagc
     3361 ttaagtaacg ccactttgca aggcatggaa aaatacataa ctgagaatag aaaagttcag
     3421 atcaaggtca ggaacaaaga aacagctgaa taccaaacag gatatctgtg gtaagcggtt
     3481 cctgccccgg ctcagggcca agaacagatg agacagctga gtgatgggcc aaacaggata
     3541 tctgtggtaa gcagttcctg ccccggctcg gggccaagaa cagatggtcc ccagatgcgg
     3601 tccagccctc agcagtttct agtgaatcat cagatgtttc cagggtgccc caaggacctg
     3661 aaaatgaccc tgtaccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     3721 cgcttccgct ctccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct
     3781 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
     3841 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga
     3901 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg
     3961 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
     4021 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg
     4081 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc
     4141 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
     4201 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
     4261 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
     4321 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
     4381 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     4441 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     4501 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     4561 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     4621 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     4681 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     4741 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt
     4801 gatcccccgg gctgcaggtc ggccgccacg accggtgccg ccaccatccc ctgacccacg
     4861 cccctgaccc ctcacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
     4921 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     4981 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     5041 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5101 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5161 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5221 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     5281 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5341 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5401 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac
     5461 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     5521 cgaaaggagc gcacgacccc atggctccga ccgaagccga cccgggcggc cccgccgacc
     5581 ccgcacccgc ccccgaggcc caccgactct agtaatagta atcaattacg gggtcattag
     5641 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5701 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5761 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5821 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5881 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5941 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     6001 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     6061 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     6121 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     6181 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagatggtga gcaagggcga
     6241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     6301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     6361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     6421 ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     6481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgc
     6661 catcagcgac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     6721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6841 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6901 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtaccatgt cttcaggaaa
     6961 tgctaaaatt gggcaccctg cccccaactt caaagccaca gctgttatgc cagatggtca
     7021 gtttaaagat atcagcctgt ctgactacaa aggaaaatat gttgtgttct tcttttaccc
     7081 tcttgacttc acctttgtgt gccccacgga gatcattgct ttcagtgata gggcagaaga
     7141 atttaagaaa ctcaactgcc aagtgattgg tgcttctgtg gattctcact tctgtcatct
     7201 agcatgggtc aatacaccta agaaacaagg aggactggga cccatgaaca ttcctttggt
     7261 atcagacccg aagcgcacca ttgctcagga ttatggggtc ttaaaggctg atgaaggcat
     7321 ctcgttcagg ggccttttta tcattgatga taagggtatt cttcggcaga tcactgtaaa
     7381 tgacctccct gttggccgct ctgtggatga gactttgaga ctagttcagg ccttccagtt
     7441 cactgacaaa catggggaag tgtgcccagc tggctggaaa cctggcagtg ataccatcaa
     7501 gcctgatgtc caaaagagca aagaatattt ctccaagcag aagtaa
//
