LOCUS       sequence-305309-       11008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing mammalian proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11008)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Puro-Ak
FEATURES             Location/Qualifiers
     source          1..11008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..1313
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          366..1304
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1261..1281
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1361..1478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1586..2153
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1604..2155
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1748..1765)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1975..1994
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2158..2757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2158..2177)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2654..2674
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2799..2815)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2800..2816)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2829..3417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2882..2902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3300..3311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3561..3594)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3614..3794
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3819..3837)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3820..3837)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4127..4146)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4360..4379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4479..4501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4539..4557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4625..4729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4730..4798,4799..5590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4948..4967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5761..6349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6250..6269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6503..6520
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6590..6610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6595..6924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6775..6910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8058..8123
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8253..8273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8698..8832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8735..8754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8789..8808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9000..9633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9680..9805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10298..10531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10716..10760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cgatcacgag actagcctcg accatcgatg tcgacgataa gctttgcaaa
       61 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
      121 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      181 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
      241 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      301 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      361 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
      421 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
      481 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      541 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
      601 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
      661 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
      721 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
      781 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
      841 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
      901 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
      961 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     1021 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     1081 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1141 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1201 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1261 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattt
     1321 cgacatttaa atttaattaa tctcgacggt atcggttaac ttttaaaaga aaagggggga
     1381 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1441 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctcg
     1501 aggtttaaac ccgggcgcgc ctgcaggatc catatgcgga ccgtacgtac tagtggccac
     1561 ggctgatcat acgggctgca ggaattccgc cccccccccc ctaacgttac tggccgaagc
     1621 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     1681 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     1741 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     1801 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     1861 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     1921 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     1981 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     2041 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc
     2101 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taataccatg
     2161 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     2221 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     2281 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     2341 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     2401 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     2461 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     2521 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     2581 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     2641 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     2701 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgg
     2761 gcgcgtctgg aacttcgaat tcgatatcaa gcttatcgcg ataccgtcga cctcgaggga
     2821 attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3241 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3361 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3421 gggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3481 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3541 gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag ttatgctgct
     3601 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3661 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3721 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3781 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     3841 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     3901 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     3961 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     4021 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     4081 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     4141 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     4201 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     4261 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     4321 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     4381 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4501 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6421 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6481 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6541 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     6601 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6661 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6721 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6781 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6841 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6901 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     6961 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     7021 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     7081 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     7141 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     7201 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     7261 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     7321 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     7381 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     7441 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     7501 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     7561 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     7621 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     7681 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     7741 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     7801 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     7861 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     7921 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     7981 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     8041 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     8101 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     8161 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     8221 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     8281 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     8341 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     8401 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     8461 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     8521 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     8581 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8641 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8821 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     8881 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     8941 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     9001 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     9061 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     9121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     9181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     9301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     9361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     9421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     9661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     9721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     9781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     9841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     9901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     9961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    10021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    10081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    10141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    10201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    10501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    10561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    10621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    10681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    10741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    10801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    10861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    10921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    10981 acagatccat tcgattagtg aacggatc
//
