LOCUS       sequence-305324-        5663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TMPRSS2 with N-terminal MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB
  TITLE     Angiotensin converting enzyme 2 is a novel target of the 
            gamma-secretase complex.
  JOURNAL   Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x.
  PUBMED    33963249
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-05-7"; volume: "11(1):9803. doi"; pages: " 
            10.1038/s41598-021-89379-x"
COMMENT     Sequence Label: pCSDest-MYC-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..5663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(103..207)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(233..688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(370..391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        759..1743
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1778..1796
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1778..1795
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1850..1874
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1880..1909
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(3390..3414)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3458..3592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3482..3501)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3536..3555
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3705..3725)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3705..3723)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3744..3760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3744..3760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3757..3779)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3768..3784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3792..3798,3799..3816,3817..3822))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3837..3858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3975..3992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4146..4734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4226..4245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4905..5663,1..33,34..102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5528..5547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
       61 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
      241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      661 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
      721 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg
      781 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
      841 cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg
      901 cggacttggc accatgccaa ttcaatatgg cggacctggc actgtgccaa ctggggaggg
      961 gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat
     1021 attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata
     1081 taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt
     1141 attggcccta tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc
     1201 tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc atagccactc
     1261 ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc
     1321 ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc
     1381 tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg
     1441 cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg
     1501 gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg gaagtacgcc
     1561 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
     1621 caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg
     1681 acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg
     1741 aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaatac
     1801 aagctacttg ttctttttgc aggatcccat cgattcgaat tcaaggatca caagtttgta
     1861 caaaaaagca ggcaccatgg aacagaaact gattagcgaa gaagatctga tggctttgaa
     1921 ctcagggtca ccaccagcta ttggacctta ctatgaaaac catggatacc aaccggaaaa
     1981 cccctatccc gcacagccca ctgtggtccc cactgtctac gaggtgcatc cggctcagta
     2041 ctacccgtcc cccgtgcccc agtacgcccc gagggtcctg acgcaggctt ccaaccccgt
     2101 cgtctgcacg cagcccaaat ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc
     2161 actgtgcatc accttgaccc tggggacctt cctcgtggga gctgcgctgg ccgctggcct
     2221 actctggaag ttcatgggca gcaagtgctc caactctggg atagagtgcg actcctcagg
     2281 tacctgcatc aacccctcta actggtgtga tggcgtgtca cactgccccg gcggggagga
     2341 cgagaatcgg tgtgttcgcc tctacggacc aaacttcatc cttcaggtgt actcatctca
     2401 gaggaagtcc tggcaccctg tgtgccaaga cgactggaac gagaactacg ggcgggcggc
     2461 ctgcagggac atgggctata agaataattt ttactctagc caaggaatag tggatgacag
     2521 cggatccacc agctttatga aactgaacac aagtgccggc aatgtcgata tctataaaaa
     2581 actgtaccac agtgatgcct gttcttcaaa agcagtggtt tctttacgct gtatagcctg
     2641 cggggtcaac ttgaactcaa gccgccagag caggatcgtg ggcggcgaga gcgcgctccc
     2701 gggggcctgg ccctggcagg tcagcctgca cgtccagaac gtccacgtgt gcggaggctc
     2761 catcatcacc cccgagtgga tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa
     2821 tccatggcat tggacggcat ttgcggggat tttgagacaa tctttcatgt tctatggagc
     2881 cggataccaa gtagaaaaag tgatttctca tccaaattat gactccaaga ccaagaacaa
     2941 tgacattgcg ctgatgaagc tgcagaagcc tctgactttc aacgacctag tgaaaccagt
     3001 gtgtctgccc aacccaggca tgatgctgca gccagaacag ctctgctgga tttccgggtg
     3061 gggggccacc gaggagaaag ggaagacctc agaagtgctg aacgctgcca aggtgcttct
     3121 cattgagaca cagagatgca acagcagata tgtctatgac aacctgatca caccagccat
     3181 gatctgtgcc ggcttcctgc aggggaacgt cgattcttgc cagggtgaca gtggagggcc
     3241 tctggtcact tcgaagaaca atatctggtg gctgataggg gatacaagct ggggttctgg
     3301 ctgtgccaaa gcttacagac caggagtgta cgggaatgtg atggtattca cggactggat
     3361 ttatcgacaa atgagggcag acggctagaa cccagctttc ttgtacaaag tggtgatcct
     3421 ctcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     3481 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3541 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3601 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     3661 ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta
     3721 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3781 acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga
     3841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5641 actcgtgcac ccaactgatc ttc
//
