LOCUS       sequence-30533-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Conaway JW, Florens L, Sato S, Tomomori-Sato C, Parmely TJ, Yao T, 
            Swanson SK, Banks CA, Washburn MP, Conaway RC
  TITLE     The mammalian Mediator complex.
  JOURNAL   FEBS Lett. 2005 Feb 7. 579(4):904-8.
  PUBMED    15680972
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2005 Feb 7. 579(4):904-8."
COMMENT     Sequence Label: F-CDK8L
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcatgccag actacaagga cgatgatgac aagctcgaga
      121 tggattatga tttcaaggcg aagctggcgg cggagcggga gcgggtggag gatttgtttg
      181 agtacgaagg gtgcaaagtg ggacgcggca cctacggtca cgtctacaag gcgaggcgga
      241 aagatggaaa agatgaaaag gaatatgcat tgaagcaaat tgaaggcaca ggaatatcca
      301 tgtcggcttg tagagagatt gcacttttgc gagaattgaa gcaccctaat gtgattgcat
      361 tgcagaaggt gttcctttct cacagtgaca ggaaggtatg gctgctgttt gattatgcag
      421 agcatgactt gtggcatatt attaagtttc accgtgcatc aaaagcaaat aaaaagccca
      481 tgcagttgcc aagatctatg gttaaatcct tactttacca gattcttgat ggtatccatt
      541 acctccatgc aaattgggtg cttcacagag acttgaaacc agcaaatatc ctagtaatgg
      601 gagaaggtcc tgagaggggg agagtcaaaa tagctgacat gggttttgcc agattattca
      661 attctcctct aaagccacta gcagatttgg atccagtagt tgtgacattt tggtatcggg
      721 ctccagaact tttgcttggt gcaaggcatt atacaaaggc cattgatata tgggcaatag
      781 gttgtatatt tgctgaattg ttgacttcgg aacctatttt tcactgtcgt caggaagata
      841 taaaaacaag caatcccttt catcatgatc aactggatcg gatatttagt gtcatggggt
      901 ttcctgcaga taaagactgg gaaga
//
