LOCUS       sequence-305330-        5660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TMPRSS2 with N-terminal HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB
  TITLE     Angiotensin converting enzyme 2 is a novel target of the 
            gamma-secretase complex.
  JOURNAL   Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x.
  PUBMED    33963249
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-05-7"; volume: "11(1):9803. doi"; pages: " 
            10.1038/s41598-021-89379-x"
COMMENT     Sequence Label: pCSDest-HA-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..5660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(103..207)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(233..688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(370..391)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..601
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        759..1743
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1778..1796
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1778..1795
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1850..1874
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1880..1906
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3387..3411)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3455..3589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3479..3498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3533..3552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3702..3722)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3702..3720)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3741..3757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3741..3757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3754..3776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3765..3781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3789..3795,3796..3813,3814..3819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3834..3855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3972..3989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4143..4731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4223..4242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4902..5660,1..33,34..102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5525..5544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
       61 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
      241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
      301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      661 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
      721 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg
      781 cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg
      841 cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg
      901 cggacttggc accatgccaa ttcaatatgg cggacctggc actgtgccaa ctggggaggg
      961 gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat
     1021 attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata
     1081 taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt
     1141 attggcccta tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc
     1201 tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc atagccactc
     1261 ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc
     1321 ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc
     1381 tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg
     1441 cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg
     1501 gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg gaagtacgcc
     1561 agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc
     1621 caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg
     1681 acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg
     1741 aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaatac
     1801 aagctacttg ttctttttgc aggatcccat cgattcgaat tcaaggatca caagtttgta
     1861 caaaaaagca ggcaccatgt atccgtatga tgtgccggat tatgcgatgg ctttgaactc
     1921 agggtcacca ccagctattg gaccttacta tgaaaaccat ggataccaac cggaaaaccc
     1981 ctatcccgca cagcccactg tggtccccac tgtctacgag gtgcatccgg ctcagtacta
     2041 cccgtccccc gtgccccagt acgccccgag ggtcctgacg caggcttcca accccgtcgt
     2101 ctgcacgcag cccaaatccc catccgggac agtgtgcacc tcaaagacta agaaagcact
     2161 gtgcatcacc ttgaccctgg ggaccttcct cgtgggagct gcgctggccg ctggcctact
     2221 ctggaagttc atgggcagca agtgctccaa ctctgggata gagtgcgact cctcaggtac
     2281 ctgcatcaac ccctctaact ggtgtgatgg cgtgtcacac tgccccggcg gggaggacga
     2341 gaatcggtgt gttcgcctct acggaccaaa cttcatcctt caggtgtact catctcagag
     2401 gaagtcctgg caccctgtgt gccaagacga ctggaacgag aactacgggc gggcggcctg
     2461 cagggacatg ggctataaga ataattttta ctctagccaa ggaatagtgg atgacagcgg
     2521 atccaccagc tttatgaaac tgaacacaag tgccggcaat gtcgatatct ataaaaaact
     2581 gtaccacagt gatgcctgtt cttcaaaagc agtggtttct ttacgctgta tagcctgcgg
     2641 ggtcaacttg aactcaagcc gccagagcag gatcgtgggc ggcgagagcg cgctcccggg
     2701 ggcctggccc tggcaggtca gcctgcacgt ccagaacgtc cacgtgtgcg gaggctccat
     2761 catcaccccc gagtggatcg tgacagccgc ccactgcgtg gaaaaacctc ttaacaatcc
     2821 atggcattgg acggcatttg cggggatttt gagacaatct ttcatgttct atggagccgg
     2881 ataccaagta gaaaaagtga tttctcatcc aaattatgac tccaagacca agaacaatga
     2941 cattgcgctg atgaagctgc agaagcctct gactttcaac gacctagtga aaccagtgtg
     3001 tctgcccaac ccaggcatga tgctgcagcc agaacagctc tgctggattt ccgggtgggg
     3061 ggccaccgag gagaaaggga agacctcaga agtgctgaac gctgccaagg tgcttctcat
     3121 tgagacacag agatgcaaca gcagatatgt ctatgacaac ctgatcacac cagccatgat
     3181 ctgtgccggc ttcctgcagg ggaacgtcga ttcttgccag ggtgacagtg gagggcctct
     3241 ggtcacttcg aagaacaata tctggtggct gataggggat acaagctggg gttctggctg
     3301 tgccaaagct tacagaccag gagtgtacgg gaatgtgatg gtattcacgg actggattta
     3361 tcgacaaatg agggcagacg gctagaaccc agctttcttg tacaaagtgg tgatcctctc
     3421 gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
     3481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3541 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3601 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     3661 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     3721 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3781 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     3841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5641 cgtgcaccca actgatcttc
//
