LOCUS       sequence-305345-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_NWS.AU1.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..123
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             178..201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcaact ggtcccaccc ccaattcgag
      121 aaaggaggag gcgggggagg ggacacctat cggtacattg gaggaggcgg gggaggggac
      181 tacaaagacg atgacgacaa gcccacaggc ctgtacacac acagcggtga gtgctgcaaa
      241 gcctgcaacc tgggcgaggg tgtggcccag ccttgtggag ccaaccagac cgtgtgtgag
      301 ccctgcctgg acagcgtgac gttctccgac gtggtgagcg cgaccgagcc gtgcaagccg
      361 tgcaccgagt gcgtggggct ccagagcatg tcggcgccgt gcgtggaggc cgacgacgcc
      421 gtgtgccgct gcgcctacgg ctactaccag gatgagacga ctgggcgctg cgaggcgtgc
      481 cgcgtgtgcg aggcgggctc gggcctcgtg ttctcctgcc aggacaagca gaacaccgtg
      541 tgcgaggagt gccccgacgg cacgtattcc gacgaggcca accacgtgga cccgtgcctg
      601 ccctgcaccg tgtgcgagga caccgagcgc cagctccgcg agtgcacacg ctgggccgac
      661 gccgagtgcg aggagatccc tggccgttgg attacacggt ccacaccccc agagggctcg
      721 gacagcacag cccccagcac ccaggagcct gaggcacctc cagaacaaga cctcatagcc
      781 agcacggtgg caggtgtggt gaccacagtg atgggcagct cccagcccgt ggtgacccga
      841 ggcaccaccg acaacctcat ccctgtctat tgctccatcc tggctgctgt ggttgtgggc
      901 cttgtggcct acatagcctt caagaggtgg aacaggggta tcctctag
//
