LOCUS       sequence-30535-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(599..617)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(600..617)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(637..654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    643..867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgactctcgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctta cggcagccaa catggactat aaggacgatg atgacaaagg
      121 atccgcaaag caaccttctg atgtaagttc tgagtgtgac cgagaaggta gacaattgca
      181 gcctgcggag aggcctcccc agctcagacc tggggcccct acctccctac agacagagcc
      241 acaagacagg agcccagcac ccatgagttg tgacaaatca acacaaaccc caagtcctcc
      301 ttgccaggcc ttcaaccact atctcagtgc aatggcttcc atgaggcagg ctgaacctgc
      361 agatatgcgc ccagagatat ggatcgccca agagttgcgg cgtatcggag acgagtttaa
      421 cgcttactat gcaaggaggg tatttttgaa taattaccaa gcagccgaag accacccacg
      481 aatggttatc ttacgactgt tacgttacat tgtccgcctg gtgtggagaa tgcattgaga
      541 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc
      601 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
      661 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      721 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      781 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
      841 atgctgggga tgcggtgggc tctatggctt ctga
//
