LOCUS       sequence-30536-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL (S44A)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(593..611)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(594..611)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(631..648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    637..861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag cttacggcag ccaacatgga ctataaggac gatgatgaca aaggatccgc
      121 aaagcaacct tctgatgtaa gttctgagtg tgaccgagaa ggtagacaat tgcagcctgc
      181 ggagaggcct ccccagctca gacctggggc ccctacctcc ctacagacag agccacaaga
      241 cagggcccca gcacccatga gttgtgacaa atcaacacaa accccaagtc ctccttgcca
      301 ggccttcaac cactatctca gtgcaatggc ttccatgagg caggctgaac ctgcagatat
      361 gcgcccagag atatggatcg cccaagagtt gcggcgtatc ggagacgagt ttaacgctta
      421 ctatgcaagg agggtatttt tgaataatta ccaagcagcc gaagaccacc cacgaatggt
      481 tatcttacga ctgttacgtt acattgtccg cctggtgtgg agaatgcatt gagaattctg
      541 cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt
      601 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
      661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      721 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      781 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      841 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
      901 atcccca
//
