LOCUS       sequence-305367-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HA.NWS.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             145..168
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             187..210
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgctacc catacgatgt gccagattac
      121 gctggaggag gcgggggagg gaactggtcc cacccccaat tcgagaaagg aggaggcggg
      181 ggaggggact acaaagacga tgacgacaag cccacaggcc tgtacacaca cagcggtgag
      241 tgctgcaaag cctgcaacct gggcgagggt gtggcccagc cttgtggagc caaccagacc
      301 gtgtgtgagc cctgcctgga cagcgtgacg ttctccgacg tggtgagcgc gaccgagccg
      361 tgcaagccgt gcaccgagtg cgtggggctc cagagcatgt cggcgccgtg cgtggaggcc
      421 gacgacgccg tgtgccgctg cgcctacggc tactaccagg atgagacgac tgggcgctgc
      481 gaggcgtgcc gcgtgtgcga ggcgggctcg ggcctcgtgt tctcctgcca ggacaagcag
      541 aacaccgtgt gcgaggagtg ccccgacggc acgtattccg acgaggccaa ccacgtggac
      601 ccgtgcctgc cctgcaccgt gtgcgaggac accgagcgcc agctccgcga gtgcacacgc
      661 tgggccgacg ccgagtgcga ggagatccct ggccgttgga ttacacggtc cacaccccca
      721 gagggctcgg acagcacagc ccccagcacc caggagcctg aggcacctcc agaacaagac
      781 ctcatagcca gcacggtggc aggtgtggtg accacagtga tgggcagctc ccagcccgtg
      841 gtgacccgag gcaccaccga caacctcatc cctgtctatt gctccatcct ggctgctgtg
      901 gttgtgggcc ttgtggccta catagccttc aagaggtgga acaggggtat cctctag
//
