LOCUS       sequence-305368-        8843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HA.V5.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..8843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        317..392
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    440..557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        697..1874
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          928..1865
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1822..1842
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1923..1932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2025..2051
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2070..2111
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2130..2153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2916..3504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2969..2989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3387..3398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3579..3812
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3884..4005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3921..3940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3975..3994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4045..4180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4200..4219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4201..4219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4229..4246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4229..4245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4238..4260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4387..4842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4474..4493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4684..4705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4868..4972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4973..5041,5042..5833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5191..5210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6004..6592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6493..6512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6746..6763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6880..6901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6916..6921,6922..6939,6940..6946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6954..6970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6959..6981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6978..6994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6978..6994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7013..7033
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7015..7033
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7061..7287
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7288..7468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7515..7640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8133..8366
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8551..8595
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cgatcacgag actagtatcg agcggccgcc cccttcaccg agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 gtcttcgaga agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 atcaacttga aaaagtggca ccgagtcggt gcttttttta attctcgacc tcgagacaaa
      421 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      481 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      541 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
      601 tcgaggcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      661 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      721 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      781 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      841 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      901 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      961 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga
     1021 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     1081 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     1141 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     1201 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1261 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1321 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     1381 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     1441 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     1501 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1561 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1621 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1681 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1741 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1801 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1861 ttcaggtgtc gtgaggaatt ctgcagtcga tcgacggtac cgcgggcccg ggatccacgg
     1921 tcgccaccat gggggcaggt gccaccggcc gcgccatgga cgggccgcgc ctgctgctgt
     1981 tgctgcttct gggggtgtcc cttggaggtg ccaaggaggc atgctaccca tacgatgtgc
     2041 cagattacgc tggaggaggc gggggagggg gcaaaccaat tccaaaccca ctgctgggac
     2101 tggattccac aggaggaggc gggggagggg actacaaaga cgatgacgac aagcccacag
     2161 gcctgtacac acacagcggt gagtgctgca aagcctgcaa cctgggcgag ggtgtggccc
     2221 agccttgtgg agccaaccag accgtgtgtg agccctgcct ggacagcgtg acgttctccg
     2281 acgtggtgag cgcgaccgag ccgtgcaagc cgtgcaccga gtgcgtgggg ctccagagca
     2341 tgtcggcgcc gtgcgtggag gccgacgacg ccgtgtgccg ctgcgcctac ggctactacc
     2401 aggatgagac gactgggcgc tgcgaggcgt gccgcgtgtg cgaggcgggc tcgggcctcg
     2461 tgttctcctg ccaggacaag cagaacaccg tgtgcgagga gtgccccgac ggcacgtatt
     2521 ccgacgaggc caaccacgtg gacccgtgcc tgccctgcac cgtgtgcgag gacaccgagc
     2581 gccagctccg cgagtgcaca cgctgggccg acgccgagtg cgaggagatc cctggccgtt
     2641 ggattacacg gtccacaccc ccagagggct cggacagcac agcccccagc acccaggagc
     2701 ctgaggcacc tccagaacaa gacctcatag ccagcacggt ggcaggtgtg gtgaccacag
     2761 tgatgggcag ctcccagccc gtggtgaccc gaggcaccac cgacaacctc atccctgtct
     2821 attgctccat cctggctgct gtggttgtgg gccttgtggc ctacatagcc ttcaagaggt
     2881 ggaacagggg tatcctctag agcggccgcg tcgacaatca acctctggat tacaaaattt
     2941 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3001 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3061 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3121 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3181 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3241 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3301 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3361 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3421 gcctgctgcc ggctctgcgg cctcttccgc atcttcgcct tcgccctcag acgagtcgga
     3481 tctccctttg ggccgcctcc ccgcctgggt acctttaaga ccaatgactt acaaggcagc
     3541 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3601 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     3661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3781 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     3841 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     3901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     4021 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4141 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     4201 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     4261 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4321 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4381 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4441 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4501 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4561 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4621 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4681 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4741 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4801 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4861 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4921 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4981 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5041 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5101 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5161 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5221 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5281 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5341 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5401 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5461 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5521 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5581 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5641 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5701 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5761 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5821 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5881 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5941 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6721 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6781 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6901 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6961 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7021 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     7081 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     7141 caccgtgcat ggcgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     7201 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     7261 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     7321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7441 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     7501 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     7561 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     7621 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7681 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7741 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7801 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7861 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7921 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7981 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8041 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8101 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8161 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     8221 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     8281 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     8341 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     8401 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     8461 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     8521 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     8581 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8641 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8701 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8761 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     8821 tccattcgat tagtgaacgg atc
//
