LOCUS       sequence-305371-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_NWS.V5.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..123
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             142..183
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             202..225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcaact ggtcccaccc ccaattcgag
      121 aaaggaggag gcgggggagg gggcaaacca attccaaacc cactgctggg actggattcc
      181 acaggaggag gcgggggagg ggactacaaa gacgatgacg acaagcccac aggcctgtac
      241 acacacagcg gtgagtgctg caaagcctgc aacctgggcg agggtgtggc ccagccttgt
      301 ggagccaacc agaccgtgtg tgagccctgc ctggacagcg tgacgttctc cgacgtggtg
      361 agcgcgaccg agccgtgcaa gccgtgcacc gagtgcgtgg ggctccagag catgtcggcg
      421 ccgtgcgtgg aggccgacga cgccgtgtgc cgctgcgcct acggctacta ccaggatgag
      481 acgactgggc gctgcgaggc gtgccgcgtg tgcgaggcgg gctcgggcct cgtgttctcc
      541 tgccaggaca agcagaacac cgtgtgcgag gagtgccccg acggcacgta ttccgacgag
      601 gccaaccacg tggacccgtg cctgccctgc accgtgtgcg aggacaccga gcgccagctc
      661 cgcgagtgca cacgctgggc cgacgccgag tgcgaggaga tccctggccg ttggattaca
      721 cggtccacac ccccagaggg ctcggacagc acagccccca gcacccagga gcctgaggca
      781 cctccagaac aagacctcat agccagcacg gtggcaggtg tggtgaccac agtgatgggc
      841 agctcccagc ccgtggtgac ccgaggcacc accgacaacc tcatccctgt ctattgctcc
      901 atcctggctg ctgtggttgt gggccttgtg gcctacatag ccttcaagag gtggaacagg
      961 ggtatcctct ag
//
