LOCUS       sequence-305377-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_NWS.AU1.C_NGFR
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..123
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             178..213
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcaact ggtcccaccc ccaattcgag
      121 aaaggaggag gcgggggagg ggacacctat cggtacattg gaggaggcgg gggaggggag
      181 gaccaggtgg accccaggct gatcgacggc aagcccacag gcctgtacac acacagcggt
      241 gagtgctgca aagcctgcaa cctgggcgag ggtgtggccc agccttgtgg agccaaccag
      301 accgtgtgtg agccctgcct ggacagcgtg acgttctccg acgtggtgag cgcgaccgag
      361 ccgtgcaagc cgtgcaccga gtgcgtgggg ctccagagca tgtcggcgcc gtgcgtggag
      421 gccgacgacg ccgtgtgccg ctgcgcctac ggctactacc aggatgagac gactgggcgc
      481 tgcgaggcgt gccgcgtgtg cgaggcgggc tcgggcctcg tgttctcctg ccaggacaag
      541 cagaacaccg tgtgcgagga gtgccccgac ggcacgtatt ccgacgaggc caaccacgtg
      601 gacccgtgcc tgccctgcac cgtgtgcgag gacaccgagc gccagctccg cgagtgcaca
      661 cgctgggccg acgccgagtg cgaggagatc cctggccgtt ggattacacg gtccacaccc
      721 ccagagggct cggacagcac agcccccagc acccaggagc ctgaggcacc tccagaacaa
      781 gacctcatag ccagcacggt ggcaggtgtg gtgaccacag tgatgggcag ctcccagccc
      841 gtggtgaccc gaggcaccac cgacaacctc atccctgtct attgctccat cctggctgct
      901 gtggttgtgg gccttgtggc ctacatagcc ttcaagaggt ggaacagggg tatcctctag
//
