LOCUS       sequence-30539-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag BimL (S58D)
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(606..624)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(607..624)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(644..661)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 atagcgagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actccccttt tgggagaccc aagcttacgg cagccaacat ggactataag gacgatgatg
      121 acaaaggatc cgcaaagcaa ccttctgatg taagttctga gtgtgaccga gaaggtagac
      181 aattgcagcc tgcggagagg cctccccagc tcagacctgg ggcccctacc tccctacaga
      241 cagagccaca agacaggagc ccagcaccca tgagttgtga caaatcaaca caaaccccag
      301 atcctccttg ccaggccttc aaccactatc tcagtgcaat ggcttccatg aggcaggctg
      361 aacctgcaga tatgcgccca gagatatgga tcgcccaaga gttgcggcgt atcggagacg
      421 agtttaacgc ttactatgca aggagggtat ttttgaataa ttaccaagca gccgaagacc
      481 acccacgaat ggttatctta cgactgttac gttacattgt ccgcctggtg tggagaatgc
      541 attgagaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc
      601 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
      661 agttgccagc catctgttgn ttgcccctcc cccgtgcctt ccttgacctg gaaggtgcca
      721 ctcccactgt cctttcctaa tatatgagga aat
//
