LOCUS       sequence-30542-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..110
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(591..609)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(592..609)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(629..646)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    635..859
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttatg gctagcatga ctggtggaca gcaaatgggt ggatccgcaa
      121 agcaaccttc tgatgtaagt tctgagtgtg accgagaagg tagacaattg cagcctgcgg
      181 agaggcctcc ccagctcaga cctggggccc ctacctccct acagacagag ccacaagaca
      241 ggagcccagc acccatgagt tgtgacaaat caacacaaac cccaagtcct ccttgccagg
      301 ccttcaacca ctatctcagt gcaatggctt ccatgaggca ggctgaacct gcagatatgc
      361 gcccagagat atggatcgcc caagagttgc ggcgtatcgg agacgagttt aacgcttact
      421 atgcaaggag ggtatttttg aataattacc aagcagccga agaccaccca cgaatggtta
      481 tcttacgact gttacgttac attgtccgcc tggtgtggag aatgcattga gaattctgca
      541 gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat tctatagtgt
      601 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      661 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      721 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      781 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      841 gatgcggtgg gctctatggc ttctgaggcg g
//
