LOCUS       sequence-305433-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.C.V5_NGFR
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..129
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             148..183
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             202..243
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgccagc ccgagctggc ccccgaggac
      121 cccgaggacg gaggaggcgg gggaggggag gaccaggtgg accccaggct gatcgacggc
      181 aagggaggag gcgggggagg gggcaaacca attccaaacc cactgctggg actggattcc
      241 acacccacag gcctgtacac acacagcggt gagtgctgca aagcctgcaa cctgggcgag
      301 ggtgtggccc agccttgtgg agccaaccag accgtgtgtg agccctgcct ggacagcgtg
      361 acgttctccg acgtggtgag cgcgaccgag ccgtgcaagc cgtgcaccga gtgcgtgggg
      421 ctccagagca tgtcggcgcc gtgcgtggag gccgacgacg ccgtgtgccg ctgcgcctac
      481 ggctactacc aggatgagac gactgggcgc tgcgaggcgt gccgcgtgtg cgaggcgggc
      541 tcgggcctcg tgttctcctg ccaggacaag cagaacaccg tgtgcgagga gtgccccgac
      601 ggcacgtatt ccgacgaggc caaccacgtg gacccgtgcc tgccctgcac cgtgtgcgag
      661 gacaccgagc gccagctccg cgagtgcaca cgctgggccg acgccgagtg cgaggagatc
      721 cctggccgtt ggattacacg gtccacaccc ccagagggct cggacagcac agcccccagc
      781 acccaggagc ctgaggcacc tccagaacaa gacctcatag ccagcacggt ggcaggtgtg
      841 gtgaccacag tgatgggcag ctcccagccc gtggtgaccc gaggcaccac cgacaacctc
      901 atccctgtct attgctccat cctggctgct gtggttgtgg gccttgtggc ctacatagcc
      961 ttcaagaggt ggaacagggg tatcctctag
//
