LOCUS       sequence-30544-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (AAA)
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(585..603)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(586..603)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(623..640)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    629..853
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tatggctagc atgactggtg gacagcaaat gggtggatcc gcaaagcaac
      121 cttctgatgt aagttctgag tgtgaccgag aaggtagaca attgcagcct gcggagaggc
      181 ctccccagct cagacctggg gcccctacct ccctacagac agagccacaa gacagggccc
      241 cagcacccat gagttgtgac aaatcaacac aagccccagc tcctccttgc caggccttca
      301 accactatct cagtgcaatg gcttccatga ggcaggctga acctgcagat atgcgcccag
      361 agatatggat cgcccaagag ttgcggcgta tcggagacga gtttaacgct tactatgcaa
      421 ggagggtatt tttgaataat taccaagcag ccgaagacca cccacgaatg gttatcttac
      481 gactgttacg ttacattgtc cgcctggtgt ggagaatgca ttgagaattc tgcagatatc
      541 catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
      601 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      661 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      721 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      781 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      841 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
      901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg tta
//
