LOCUS       sequence-30545-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (S44A)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             84..116
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(597..615)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(598..615)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(635..652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agcagactct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcnctttngg gagacccaag cttatggcta gcatgactgg tggacagcaa atgggtggat
      121 ccgcaaagca accttctgat gtaagttctg agtgtgaccg agaaggtaga caattgcagc
      181 ctgcggagag gcctccccag ctcagacctg gggcccctac ctccctacag acagagccac
      241 aagacagggc cccagcaccc atgagttgtg acaaatcaac acaaacccca agtcctcctt
      301 gccaggcctt caaccactat ctcagtgcaa tggcttccat gaggcaggct gaacctgcag
      361 atatgcgccc agagatatgg atcgcccaag agttgcggcg tatcggagac gagtttaacg
      421 cttactatgc aaggagggta tttttgaata attaccaagc agccgaagac cacccacgaa
      481 tggttatctt acgactgtta cgttacattg tccgcctggt gtggagaatg cattgagaat
      541 tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg ccctattcta
      601 tagtgtcacc taaatgctag agctcgctga tcatcctcga ctgtgccttc tagttgccag
      661 ccatctgttg tttgcccctc ccccgtgccc ttccttgacc ctgtaaaggt gccactccca
      721 ctgtcctttc ctaataaaat gatgaaattg c
//
