LOCUS       sequence-305455-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.FLAG.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..129
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             193..216
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgccagc ccgagctggc ccccgaggac
      121 cccgaggacg gaggaggcgg gggaggggac tacaaagacg atgacgacaa gggaggaggc
      181 gggggaggga actggtccca cccccaattc gagaaaccca caggcctgta cacacacagc
      241 ggtgagtgct gcaaagcctg caacctgggc gagggtgtgg cccagccttg tggagccaac
      301 cagaccgtgt gtgagccctg cctggacagc gtgacgttct ccgacgtggt gagcgcgacc
      361 gagccgtgca agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc gccgtgcgtg
      421 gaggccgacg acgccgtgtg ccgctgcgcc tacggctact accaggatga gacgactggg
      481 cgctgcgagg cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc ctgccaggac
      541 aagcagaaca ccgtgtgcga ggagtgcccc gacggcacgt attccgacga ggccaaccac
      601 gtggacccgt gcctgccctg caccgtgtgc gaggacaccg agcgccagct ccgcgagtgc
      661 acacgctggg ccgacgccga gtgcgaggag atccctggcc gttggattac acggtccaca
      721 cccccagagg gctcggacag cacagccccc agcacccagg agcctgaggc acctccagaa
      781 caagacctca tagccagcac ggtggcaggt gtggtgacca cagtgatggg cagctcccag
      841 cccgtggtga cccgaggcac caccgacaac ctcatccctg tctattgctc catcctggct
      901 gctgtggttg tgggccttgt ggcctacata gccttcaaga ggtggaacag gggtatcctc
      961 tag
//
