LOCUS       sequence-305459-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.AU1.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..129
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             184..207
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgccagc ccgagctggc ccccgaggac
      121 cccgaggacg gaggaggcgg gggaggggac acctatcggt acattggagg aggcggggga
      181 ggggactaca aagacgatga cgacaagccc acaggcctgt acacacacag cggtgagtgc
      241 tgcaaagcct gcaacctggg cgagggtgtg gcccagcctt gtggagccaa ccagaccgtg
      301 tgtgagccct gcctggacag cgtgacgttc tccgacgtgg tgagcgcgac cgagccgtgc
      361 aagccgtgca ccgagtgcgt ggggctccag agcatgtcgg cgccgtgcgt ggaggccgac
      421 gacgccgtgt gccgctgcgc ctacggctac taccaggatg agacgactgg gcgctgcgag
      481 gcgtgccgcg tgtgcgaggc gggctcgggc ctcgtgttct cctgccagga caagcagaac
      541 accgtgtgcg aggagtgccc cgacggcacg tattccgacg aggccaacca cgtggacccg
      601 tgcctgccct gcaccgtgtg cgaggacacc gagcgccagc tccgcgagtg cacacgctgg
      661 gccgacgccg agtgcgagga gatccctggc cgttggatta cacggtccac acccccagag
      721 ggctcggaca gcacagcccc cagcacccag gagcctgagg cacctccaga acaagacctc
      781 atagccagca cggtggcagg tgtggtgacc acagtgatgg gcagctccca gcccgtggtg
      841 acccgaggca ccaccgacaa cctcatccct gtctattgct ccatcctggc tgctgtggtt
      901 gtgggccttg tggcctacat agccttcaag aggtggaaca ggggtatcct ctag
//
