LOCUS       sequence-30546-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (T56A)
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..102
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(583..601)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(584..601)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(621..638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    627..851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctta tggctagcat gactggtgga cagcaaatgg gtggatccgc aaagcaacct
      121 tctgatgtaa gttctgagtg tgaccgagaa ggtagacaat tgcagcctgc ggagaggcct
      181 ccccagctca gacctggggc ccctacctcc ctacagacag agccacaaga caggagccca
      241 gcacccatga gttgtgacaa atcaacacaa gccccaagtc ctccttgcca ggccttcaac
      301 cactatctca gtgcaatggc ttccatgagg caggctgaac ctgcagatat gcgcccagag
      361 atatggatcg cccaagagtt gcggcgtatc ggagacgagt ttaacgctta ctatgcaagg
      421 agggtatttt tgaataatta ccaagcagcc gaagaccacc cacgaatggt tatcttacga
      481 ctgttacgtt acattgtccg cctggtgtgg agaatgcatt gagaattctg cagatatcca
      541 tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa
      601 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      661 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      721 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      781 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
      841 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
      901 gccctgtagc ggc
//
