LOCUS       sequence-305461-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.FLAG.VSVg_NGFR
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..129
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             190..222
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgccagc ccgagctggc ccccgaggac
      121 cccgaggacg gaggaggcgg gggaggggac tacaaagacg atgacgacaa gggaggaggc
      181 gggggagggt atacagatat cgaaatgaat agactcggaa agcccacagg cctgtacaca
      241 cacagcggtg agtgctgcaa agcctgcaac ctgggcgagg gtgtggccca gccttgtgga
      301 gccaaccaga ccgtgtgtga gccctgcctg gacagcgtga cgttctccga cgtggtgagc
      361 gcgaccgagc cgtgcaagcc gtgcaccgag tgcgtggggc tccagagcat gtcggcgccg
      421 tgcgtggagg ccgacgacgc cgtgtgccgc tgcgcctacg gctactacca ggatgagacg
      481 actgggcgct gcgaggcgtg ccgcgtgtgc gaggcgggct cgggcctcgt gttctcctgc
      541 caggacaagc agaacaccgt gtgcgaggag tgccccgacg gcacgtattc cgacgaggcc
      601 aaccacgtgg acccgtgcct gccctgcacc gtgtgcgagg acaccgagcg ccagctccgc
      661 gagtgcacac gctgggccga cgccgagtgc gaggagatcc ctggccgttg gattacacgg
      721 tccacacccc cagagggctc ggacagcaca gcccccagca cccaggagcc tgaggcacct
      781 ccagaacaag acctcatagc cagcacggtg gcaggtgtgg tgaccacagt gatgggcagc
      841 tcccagcccg tggtgacccg aggcaccacc gacaacctca tccctgtcta ttgctccatc
      901 ctggctgctg tggttgtggg ccttgtggcc tacatagcct tcaagaggtg gaacaggggt
      961 atcctctag
//
