LOCUS       sequence-30547-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 BimL (S58A)
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..108
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(589..607)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(590..607)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(627..644)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    633..744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg actcccttta
       61 gggagaccca agcttatggc tagcatgact ggtggacagc aaatgggtgg atccgcaaag
      121 caaccttctg atgtaagttc tgagtgtgac cgagaaggta gacaattgca gcctgcggag
      181 aggcctcccc agctcagacc tggggcccct acctccctac agacagagcc acaagacagg
      241 agcccagcac ccatgagttg tgacaaatca acacaaaccc cagctcctcc ttgccaggcc
      301 ttcaaccact atctcagtgc aatggcttcc atgaggcagg ctgaacctgc agatatgcgc
      361 ccagagatat ggatcgccca agagttgcgg cgtatcggag acgagtttaa cgcttactat
      421 gcaaggaggg tatttttgaa taattaccaa gcagccgaag accacccacg aatggttatc
      481 ttacgactgt tacgttacat tgtccgcctg gtgtggagaa tgcattgaga attctgcaga
      541 tagccatcac actggcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca
      601 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      661 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      721 ctaataaaat gaggaaattg catcgcatt
//
