LOCUS       sequence-305475-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Tag.C.S.Ollas_NGFR
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..132
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             151..195
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             214..255
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcgagg accaggtgga ccccaggctg
      121 atcgacggca agggaggagg cgggggaggg aaggagaccg ccgccgccaa gttcgagagg
      181 cagcacatgg acagcggagg aggcggggga gggagcggct tcgccaacga gctgggcccc
      241 aggctgatgg gcaagtgccc cacaggcctg tacacacaca gcggtgagtg ctgcaaagcc
      301 tgcaacctgg gcgagggtgt ggcccagcct tgtggagcca accagaccgt gtgtgagccc
      361 tgcctggaca gcgtgacgtt ctccgacgtg gtgagcgcga ccgagccgtg caagccgtgc
      421 accgagtgcg tggggctcca gagcatgtcg gcgccgtgcg tggaggccga cgacgccgtg
      481 tgccgctgcg cctacggcta ctaccaggat gagacgactg ggcgctgcga ggcgtgccgc
      541 gtgtgcgagg cgggctcggg cctcgtgttc tcctgccagg acaagcagaa caccgtgtgc
      601 gaggagtgcc ccgacggcac gtattccgac gaggccaacc acgtggaccc gtgcctgccc
      661 tgcaccgtgt gcgaggacac cgagcgccag ctccgcgagt gcacacgctg ggccgacgcc
      721 gagtgcgagg agatccctgg ccgttggatt acacggtcca cacccccaga gggctcggac
      781 agcacagccc ccagcaccca ggagcctgag gcacctccag aacaagacct catagccagc
      841 acggtggcag gtgtggtgac cacagtgatg ggcagctccc agcccgtggt gacccgaggc
      901 accaccgaca acctcatccc tgtctattgc tccatcctgg ctgctgtggt tgtgggcctt
      961 gtggcctaca tagccttcaa gaggtggaac aggggtatcc tctag
//
