LOCUS       sequence-305481-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Tag.NWS.S.Ollas_NGFR
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..123
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             142..186
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             205..246
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcaact ggtcccaccc ccaattcgag
      121 aaaggaggag gcgggggagg gaaggagacc gccgccgcca agttcgagag gcagcacatg
      181 gacagcggag gaggcggggg agggagcggc ttcgccaacg agctgggccc caggctgatg
      241 ggcaagtgcc ccacaggcct gtacacacac agcggtgagt gctgcaaagc ctgcaacctg
      301 ggcgagggtg tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc ctgcctggac
      361 agcgtgacgt tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg caccgagtgc
      421 gtggggctcc agagcatgtc ggcgccgtgc gtggaggccg acgacgccgt gtgccgctgc
      481 gcctacggct actaccagga tgagacgact gggcgctgcg aggcgtgccg cgtgtgcgag
      541 gcgggctcgg gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg cgaggagtgc
      601 cccgacggca cgtattccga cgaggccaac cacgtggacc cgtgcctgcc ctgcaccgtg
      661 tgcgaggaca ccgagcgcca gctccgcgag tgcacacgct gggccgacgc cgagtgcgag
      721 gagatccctg gccgttggat tacacggtcc acacccccag agggctcgga cagcacagcc
      781 cccagcaccc aggagcctga ggcacctcca gaacaagacc tcatagccag cacggtggca
      841 ggtgtggtga ccacagtgat gggcagctcc cagcccgtgg tgacccgagg caccaccgac
      901 aacctcatcc ctgtctattg ctccatcctg gctgctgtgg ttgtgggcct tgtggcctac
      961 atagccttca agaggtggaa caggggtatc ctctag
//
