LOCUS       sequence-305489-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.C.V5_NGFR
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..195
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             214..255
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg aggaccaggt ggaccccagg
      181 ctgatcgacg gcaagggagg aggcggggga gggggcaaac caattccaaa cccactgctg
      241 ggactggatt ccacacccac aggcctgtac acacacagcg gtgagtgctg caaagcctgc
      301 aacctgggcg agggtgtggc ccagccttgt ggagccaacc agaccgtgtg tgagccctgc
      361 ctggacagcg tgacgttctc cgacgtggtg agcgcgaccg agccgtgcaa gccgtgcacc
      421 gagtgcgtgg ggctccagag catgtcggcg ccgtgcgtgg aggccgacga cgccgtgtgc
      481 cgctgcgcct acggctacta ccaggatgag acgactgggc gctgcgaggc gtgccgcgtg
      541 tgcgaggcgg gctcgggcct cgtgttctcc tgccaggaca agcagaacac cgtgtgcgag
      601 gagtgccccg acggcacgta ttccgacgag gccaaccacg tggacccgtg cctgccctgc
      661 accgtgtgcg aggacaccga gcgccagctc cgcgagtgca cacgctgggc cgacgccgag
      721 tgcgaggaga tccctggccg ttggattaca cggtccacac ccccagaggg ctcggacagc
      781 acagccccca gcacccagga gcctgaggca cctccagaac aagacctcat agccagcacg
      841 gtggcaggtg tggtgaccac agtgatgggc agctcccagc ccgtggtgac ccgaggcacc
      901 accgacaacc tcatccctgt ctattgctcc atcctggctg ctgtggttgt gggccttgtg
      961 gcctacatag ccttcaagag gtggaacagg ggtatcctct ag
//
