LOCUS       sequence-305491-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.C.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..195
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             214..237
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg aggaccaggt ggaccccagg
      181 ctgatcgacg gcaagggagg aggcggggga ggggactaca aagacgatga cgacaagccc
      241 acaggcctgt acacacacag cggtgagtgc tgcaaagcct gcaacctggg cgagggtgtg
      301 gcccagcctt gtggagccaa ccagaccgtg tgtgagccct gcctggacag cgtgacgttc
      361 tccgacgtgg tgagcgcgac cgagccgtgc aagccgtgca ccgagtgcgt ggggctccag
      421 agcatgtcgg cgccgtgcgt ggaggccgac gacgccgtgt gccgctgcgc ctacggctac
      481 taccaggatg agacgactgg gcgctgcgag gcgtgccgcg tgtgcgaggc gggctcgggc
      541 ctcgtgttct cctgccagga caagcagaac accgtgtgcg aggagtgccc cgacggcacg
      601 tattccgacg aggccaacca cgtggacccg tgcctgccct gcaccgtgtg cgaggacacc
      661 gagcgccagc tccgcgagtg cacacgctgg gccgacgccg agtgcgagga gatccctggc
      721 cgttggatta cacggtccac acccccagag ggctcggaca gcacagcccc cagcacccag
      781 gagcctgagg cacctccaga acaagacctc atagccagca cggtggcagg tgtggtgacc
      841 acagtgatgg gcagctccca gcccgtggtg acccgaggca ccaccgacaa cctcatccct
      901 gtctattgct ccatcctggc tgctgtggtt gtgggccttg tggcctacat agccttcaag
      961 aggtggaaca ggggtatcct ctag
//
