LOCUS       sequence-305493-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.C.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..195
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             217..240
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg aggaccaggt ggaccccagg
      181 ctgatcgacg gcaagggagg aggcggggga gggaactggt cccaccccca attcgagaaa
      241 cccacaggcc tgtacacaca cagcggtgag tgctgcaaag cctgcaacct gggcgagggt
      301 gtggcccagc cttgtggagc caaccagacc gtgtgtgagc cctgcctgga cagcgtgacg
      361 ttctccgacg tggtgagcgc gaccgagccg tgcaagccgt gcaccgagtg cgtggggctc
      421 cagagcatgt cggcgccgtg cgtggaggcc gacgacgccg tgtgccgctg cgcctacggc
      481 tactaccagg atgagacgac tgggcgctgc gaggcgtgcc gcgtgtgcga ggcgggctcg
      541 ggcctcgtgt tctcctgcca ggacaagcag aacaccgtgt gcgaggagtg ccccgacggc
      601 acgtattccg acgaggccaa ccacgtggac ccgtgcctgc cctgcaccgt gtgcgaggac
      661 accgagcgcc agctccgcga gtgcacacgc tgggccgacg ccgagtgcga ggagatccct
      721 ggccgttgga ttacacggtc cacaccccca gagggctcgg acagcacagc ccccagcacc
      781 caggagcctg aggcacctcc agaacaagac ctcatagcca gcacggtggc aggtgtggtg
      841 accacagtga tgggcagctc ccagcccgtg gtgacccgag gcaccaccga caacctcatc
      901 cctgtctatt gctccatcct ggctgctgtg gttgtgggcc ttgtggccta catagccttc
      961 aagaggtgga acaggggtat cctctag
//
