LOCUS       sequence-305501-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.V5.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..201
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg gcaaaccaat tccaaaccca
      181 ctgctgggac tggattccac aggaggaggc gggggagggg actacaaaga cgatgacgac
      241 aagcccacag gcctgtacac acacagcggt gagtgctgca aagcctgcaa cctgggcgag
      301 ggtgtggccc agccttgtgg agccaaccag accgtgtgtg agccctgcct ggacagcgtg
      361 acgttctccg acgtggtgag cgcgaccgag ccgtgcaagc cgtgcaccga gtgcgtgggg
      421 ctccagagca tgtcggcgcc gtgcgtggag gccgacgacg ccgtgtgccg ctgcgcctac
      481 ggctactacc aggatgagac gactgggcgc tgcgaggcgt gccgcgtgtg cgaggcgggc
      541 tcgggcctcg tgttctcctg ccaggacaag cagaacaccg tgtgcgagga gtgccccgac
      601 ggcacgtatt ccgacgaggc caaccacgtg gacccgtgcc tgccctgcac cgtgtgcgag
      661 gacaccgagc gccagctccg cgagtgcaca cgctgggccg acgccgagtg cgaggagatc
      721 cctggccgtt ggattacacg gtccacaccc ccagagggct cggacagcac agcccccagc
      781 acccaggagc ctgaggcacc tccagaacaa gacctcatag ccagcacggt ggcaggtgtg
      841 gtgaccacag tgatgggcag ctcccagccc gtggtgaccc gaggcaccac cgacaacctc
      901 atccctgtct attgctccat cctggctgct gtggttgtgg gccttgtggc ctacatagcc
      961 ttcaagaggt ggaacagggg tatcctctag
//
