LOCUS       sequence-305503-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.V5.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..201
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             223..246
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg gcaaaccaat tccaaaccca
      181 ctgctgggac tggattccac aggaggaggc gggggaggga actggtccca cccccaattc
      241 gagaaaccca caggcctgta cacacacagc ggtgagtgct gcaaagcctg caacctgggc
      301 gagggtgtgg cccagccttg tggagccaac cagaccgtgt gtgagccctg cctggacagc
      361 gtgacgttct ccgacgtggt gagcgcgacc gagccgtgca agccgtgcac cgagtgcgtg
      421 gggctccaga gcatgtcggc gccgtgcgtg gaggccgacg acgccgtgtg ccgctgcgcc
      481 tacggctact accaggatga gacgactggg cgctgcgagg cgtgccgcgt gtgcgaggcg
      541 ggctcgggcc tcgtgttctc ctgccaggac aagcagaaca ccgtgtgcga ggagtgcccc
      601 gacggcacgt attccgacga ggccaaccac gtggacccgt gcctgccctg caccgtgtgc
      661 gaggacaccg agcgccagct ccgcgagtgc acacgctggg ccgacgccga gtgcgaggag
      721 atccctggcc gttggattac acggtccaca cccccagagg gctcggacag cacagccccc
      781 agcacccagg agcctgaggc acctccagaa caagacctca tagccagcac ggtggcaggt
      841 gtggtgacca cagtgatggg cagctcccag cccgtggtga cccgaggcac caccgacaac
      901 ctcatccctg tctattgctc catcctggct gctgtggttg tgggccttgt ggcctacata
      961 gccttcaaga ggtggaacag gggtatcctc tag
//
