LOCUS       sequence-305511-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.FLAG.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             205..228
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcagcg gcttcgccaa cgagctgggc
      121 cccaggctga tgggcaagtg cggaggaggc gggggagggg actacaaaga cgatgacgac
      181 aagggaggag gcgggggagg gaactggtcc cacccccaat tcgagaaacc cacaggcctg
      241 tacacacaca gcggtgagtg ctgcaaagcc tgcaacctgg gcgagggtgt ggcccagcct
      301 tgtggagcca accagaccgt gtgtgagccc tgcctggaca gcgtgacgtt ctccgacgtg
      361 gtgagcgcga ccgagccgtg caagccgtgc accgagtgcg tggggctcca gagcatgtcg
      421 gcgccgtgcg tggaggccga cgacgccgtg tgccgctgcg cctacggcta ctaccaggat
      481 gagacgactg ggcgctgcga ggcgtgccgc gtgtgcgagg cgggctcggg cctcgtgttc
      541 tcctgccagg acaagcagaa caccgtgtgc gaggagtgcc ccgacggcac gtattccgac
      601 gaggccaacc acgtggaccc gtgcctgccc tgcaccgtgt gcgaggacac cgagcgccag
      661 ctccgcgagt gcacacgctg ggccgacgcc gagtgcgagg agatccctgg ccgttggatt
      721 acacggtcca cacccccaga gggctcggac agcacagccc ccagcaccca ggagcctgag
      781 gcacctccag aacaagacct catagccagc acggtggcag gtgtggtgac cacagtgatg
      841 ggcagctccc agcccgtggt gacccgaggc accaccgaca acctcatccc tgtctattgc
      901 tccatcctgg ctgctgtggt tgtgggcctt gtggcctaca tagccttcaa gaggtggaac
      961 aggggtatcc tctag
//
