LOCUS       sequence-305515-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_AU1.Ollas.FLAG_NGFR
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..174
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             196..219
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcgaca cctatcggta cattggagga
      121 ggcgggggag ggagcggctt cgccaacgag ctgggcccca ggctgatggg caagtgcgga
      181 ggaggcgggg gaggggacta caaagacgat gacgacaagc ccacaggcct gtacacacac
      241 agcggtgagt gctgcaaagc ctgcaacctg ggcgagggtg tggcccagcc ttgtggagcc
      301 aaccagaccg tgtgtgagcc ctgcctggac agcgtgacgt tctccgacgt ggtgagcgcg
      361 accgagccgt gcaagccgtg caccgagtgc gtggggctcc agagcatgtc ggcgccgtgc
      421 gtggaggccg acgacgccgt gtgccgctgc gcctacggct actaccagga tgagacgact
      481 gggcgctgcg aggcgtgccg cgtgtgcgag gcgggctcgg gcctcgtgtt ctcctgccag
      541 gacaagcaga acaccgtgtg cgaggagtgc cccgacggca cgtattccga cgaggccaac
      601 cacgtggacc cgtgcctgcc ctgcaccgtg tgcgaggaca ccgagcgcca gctccgcgag
      661 tgcacacgct gggccgacgc cgagtgcgag gagatccctg gccgttggat tacacggtcc
      721 acacccccag agggctcgga cagcacagcc cccagcaccc aggagcctga ggcacctcca
      781 gaacaagacc tcatagccag cacggtggca ggtgtggtga ccacagtgat gggcagctcc
      841 cagcccgtgg tgacccgagg caccaccgac aacctcatcc ctgtctattg ctccatcctg
      901 gctgctgtgg ttgtgggcct tgtggcctac atagccttca agaggtggaa caggggtatc
      961 ctctag
//
