LOCUS       sequence-305517-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_FLAG.Ollas.VSVg_NGFR
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             139..180
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             202..234
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcgact acaaagacga tgacgacaag
      121 ggaggaggcg ggggagggag cggcttcgcc aacgagctgg gccccaggct gatgggcaag
      181 tgcggaggag gcgggggagg gtatacagat atcgaaatga atagactcgg aaagcccaca
      241 ggcctgtaca cacacagcgg tgagtgctgc aaagcctgca acctgggcga gggtgtggcc
      301 cagccttgtg gagccaacca gaccgtgtgt gagccctgcc tggacagcgt gacgttctcc
      361 gacgtggtga gcgcgaccga gccgtgcaag ccgtgcaccg agtgcgtggg gctccagagc
      421 atgtcggcgc cgtgcgtgga ggccgacgac gccgtgtgcc gctgcgccta cggctactac
      481 caggatgaga cgactgggcg ctgcgaggcg tgccgcgtgt gcgaggcggg ctcgggcctc
      541 gtgttctcct gccaggacaa gcagaacacc gtgtgcgagg agtgccccga cggcacgtat
      601 tccgacgagg ccaaccacgt ggacccgtgc ctgccctgca ccgtgtgcga ggacaccgag
      661 cgccagctcc gcgagtgcac acgctgggcc gacgccgagt gcgaggagat ccctggccgt
      721 tggattacac ggtccacacc cccagagggc tcggacagca cagcccccag cacccaggag
      781 cctgaggcac ctccagaaca agacctcata gccagcacgg tggcaggtgt ggtgaccaca
      841 gtgatgggca gctcccagcc cgtggtgacc cgaggcacca ccgacaacct catccctgtc
      901 tattgctcca tcctggctgc tgtggttgtg ggccttgtgg cctacatagc cttcaagagg
      961 tggaacaggg gtatcctcta g
//
