LOCUS       sequence-30553-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 T7 Bmf
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..106
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(725..743)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(726..743)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(763..780)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag cttatggcta gcatgactgg tggacagcaa atgggtggat ccgagccatc
      121 tcagtgtgtg gaggagctgg aggatgatgt gttccaacca gaggatgggg agccggtgac
      181 ccaacccggg agcttgctct ctgctgacct gtttgcccag agcctactgg actgccccct
      241 cagccgactt cagctcttcc ctctcaccca ctgctgtggc cctggccttc gacccaccag
      301 ccaggaagac aaagctaccc agactctcag cccagcctcc cccagccaag gtgtcatgct
      361 gccttgtggg gtgactgagg aaccccagcg actcttttat ggcaatgctg gctatcggct
      421 tcctctccct gccagtttcc cagcagtctt gcccattggg gagcagcccc ccgaagggca
      481 gtggcaacat caagcagagg tacagattgc ccgaaagctt cagtgcattg cagaccagtt
      541 ccaccggctt catgtgcagc aacaccagca gaaccaaaat cgtgtgtggt ggcagatcct
      601 cctcttcctg cacaaccttg ctttgaatgg agaagagaac aggaacgggg caggccctag
      661 gtgagaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
      721 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      781 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      841 ctcccactgt cctttcctaa taaaatga
//
