LOCUS       sequence-30554-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag Bmf (S74A)
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(745..763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attagcagag ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttacg gcagccaaca tggactataa ggacgatgat
      121 gacaaaggat ccgagccatc tcagtgtgtg gaggagctgg aggatgatgt gttccaacca
      181 gaggatgggg agccggtgac ccaacccggg agcttgctct ctgctgacct gtttgcccag
      241 agcctactgg actgccccct cagccgactt cagctcttcc ctctcaccca ctgctgtggc
      301 cctggccttc gacccaccag ccaggaagac aaagctaccc agactctcgc cccagcctcc
      361 cccagccaag gtgtcatgct gccttgtggg gtgactgagg aaccccagcg actcttttat
      421 ggcaatgctg gctatcggct tcctctccct gccagtttcc cagcagtctt gcccattggg
      481 gagcagcccc ccgaagggca gtggcaacat caagcagagg tacagattgc ccgaaagctt
      541 cagtgcattg cagaccagtt ccaccggctt catgtgcagc aacaccagca gaaccaaaat
      601 cgtgtgtggt ggcagatcct cctcttcctg cacaaccttg ctttgaatgg agaagagaac
      661 aggaacgggg caggccctag gtgagaattc tgcagatatc catcacactg gcggccgctc
      721 gagcatgcat ctagagggcc ctnttctata gtgtcaccta aatgctagag ctcgctgatc
      781 agcctcg
//
