LOCUS       sequence-30555-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Lei K, Davis RJ
  TITLE     JNK phosphorylation of Bim-related members of the Bcl2 family 
            induces Bax-dependent apoptosis.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.
  PUBMED    12591950
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 4. 100(5):2432-7."
COMMENT     Sequence Label: pCDNA3 Flag Bmf (S74D)
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(743..761)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(744..761)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(781..798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttacggc agccaacatg gactataagg acgatgatga
      121 caaaggatcc gagccatctc agtgtgtgga ggagctggag gatgatgtgt tccaaccaga
      181 ggatggggag ccggtgaccc aacccgggag cttgctctct gctgacctgt ttgcccagag
      241 cctactggac tgccccctca gccgacttca gctcttccct ctcacccact gctgtggccc
      301 tggccttcga cccaccagcc aggaagacaa agctacccag actctcgacc cagcctcccc
      361 cagccaaggt gtcatgctgc cttgtggggt gactgaggaa ccccagcgac tcttttatgg
      421 caatgctggc tatcggcttc ctctccctgc cagtttccca gcagtcttgc ccattgggga
      481 gcagcccccc gaagggcagt ggcaacatca agcagaggta cagattgccc gaaagcttca
      541 gtgcattgca gaccagttcc accggcttca tgtgcagcaa caccagcaga accaaaatcg
      601 tgtgtggtgg cagatcctcc tcttcctgca caaccttgct ttgaatggag aagagaacag
      661 gaacggggca ggccctaggt gagaattctg cagatatcca tcacactggc ggccgctcga
      721 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
      781 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      841 tgaccctgga a
//
