LOCUS       sequence-305573-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK18 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK18_IDG-K
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(124..144)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..927
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWXGGXISLRSAEK"
ORIGIN
        1 gttttgtgag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
       61 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
      121 ggccaacttt tgtatacaaa gtgggccacc atgggaatcc cgctgctgaa cgctaaacag
      181 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat
      241 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat
      301 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg
      361 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc
      421 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa
      481 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc
      541 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg
      601 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac
      661 ctggacagga atactctggc cgctatgctg atccgagagc tgcgggcagc cctggaactg
      721 ttcgagcagg aaggcctggc tccatatctg tcacggtggg agaagctgga taacttcatc
      781 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt
      841 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gntgggcgga
      901 naaatcagtc tgcggtctgc cgaaaaggct agcggcaagc ccatccc
//
