LOCUS       sequence-305574-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK18 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK18_IDG-K
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatga acaagatgaa
      181 gaactttaag cgccgtttct ccctgtcagt gccccgcact gagaccattg aagaatcctt
      241 ggctgaattc acggagcaat tcaaccagct ccacaaccgg cggaatgaga acttgcagct
      301 cggtcctctt ggcagagacc ccccgcagga gtgcagcacc ttctccccaa cagacagcgg
      361 ggaggagccg gggcagctct cccctggcgt gcagttccag cggcggcaga accagcgccg
      421 cttctccatg gaggacgtca gcaagaggct ctctctgccc atggatatcc gcctgcccca
      481 ggaattccta cagaagctac agatggagag cccagatctg cccaagccgc tcagccgcat
      541 gtcccgccgg gcctccctgt cagacattgg ctttgggaaa ctggaaacat acgtgaaact
      601 ggacaaactg ggagagggca cctatgccac agtcttcaaa gggcgcagca aactgatgga
      661 gaaccttgtg gccctgaaag agatccggct ggagcacgag gagggagcgc cctgcactgc
      721 catccgagag gtgtctctgc tgaagaacct gaagcacgcc aatattgtga ccctgcatga
      781 cctcatccac acagatcggt ccctcaccct ggtgtttgag tacctggaca gtgacctgaa
      841 gcagtatctg gaccactgtg ggaacctcat gagcatgcac aacgtcaaga ttttcatgtt
      901 ccagctgctc cggggcctcg cctactgtca ccaccgcaag atcctgcacc gggacctgaa
      961 gcccca
//
