LOCUS       sequence-305580-       12248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of DSTYK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12248)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DSTYK _IDG-K
FEATURES             Location/Qualifiers
     source          1..12248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5403..5423)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5424..5433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5436..6206
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6213..6254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6255..6279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9076..9100)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9130..9718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9183..9203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9601..9612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9732..10231
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9767..9790)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10164..10183
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10252..10851
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10252..10271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10748..10768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10989..11222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11294..11415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11331..11350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11385..11404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11434..11569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11601..11620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11602..11620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11630..11647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11630..11646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11639..11661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11791..12246
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11878..11897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12088..12109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaagt ttgtacaaaa aagcaggctc caccatggag ggcgacgggg
     6301 tgccatgggg cagcgagccc gtctcgggtc ccggccccgg cggcggcgga atgatccgcg
     6361 agctgtgccg gggcttcggc cgctaccgcc gctacctggg acggctgcga cagaacctgc
     6421 gcgagaccca gaagttcttc cgcgacatca agtgctccca caaccacact tgtctctcct
     6481 ccctcacggg cggcggcggg gccgagcgcg gccctgcagg cgatgtcgcc gaaaccgggc
     6541 tgcaggcggg ccaactgagc tgcatttcct tcccacctaa ggaagagaag tacctccagc
     6601 agattgtgga ctgcctccct tgcatactga tcctcggcca ggattgtaac gtcaagtgcc
     6661 agctgttgaa tctgctgttg ggggtgcagg tgcttcccac caccaagctg ggcagtgagg
     6721 agagctgtaa gcttcggcgc ctccgcttca cctatgggac tcagactcgg gtcagcctgg
     6781 cgctccctgg acagtatgaa ctagtgcaca cgctggttgc tcatcagggc aactgggaga
     6841 ccatccctga ggaggatctg gaggtccaag agaacaatga ggatgctgtt catgttttag
     6901 cggaactgga ggtaacgatg caccatgctc tcttacagga agtggacgtt gtggtagcac
     6961 catgccaagg cctccggccc acagtggatg ttctgggtga cttggtgaat gatttcttgc
     7021 ctgtgataac ctatgcactc cacaaagatg aactctctga gagggatgag caagagcttc
     7081 aggaaatccg aaagtatttc tcctttcctg tattcttttt caaagtgccg aaactgggct
     7141 cggagataat agactcctca accaggagaa tggagagcga aagatcaccg ctttatcgcc
     7201 agctaattga cctgggctat ctgagcagca gtcactggaa ctgtggggct cctggccagg
     7261 atactaaagc tcagagcatg ttggtggaac agagtgaaaa gctgagacac ttgagcacat
     7321 tttctcacca ggtgttacag actcgcctgg tggatgcagc caaggccctg aacctggtgc
     7381 actgccactg ccttgacatc tttattaacc aggcatttga catgcagcgg gacctgcaga
     7441 tcactcccaa acgtctggaa tatactcgaa aaaaggagaa tgagttgtat gaatcattga
     7501 tgaatattgc caaccgaaag caggaggaaa tgaaggatat gattgttgag acacttaata
     7561 ccatgaagga ggaacttctg gatgatgcta ctaacatgga gtttaaagac gtcattgtcc
     7621 ctgagaatgg agaaccagta ggcaccagag agatcaaatg ctgcatccga cagatccagg
     7681 aactcatcat ctcccgactt aatcaggcag tggctaataa gctgatcagc tcagtggatt
     7741 acctgaggga aagcttcgtc ggaaccctgg aacgatgtct gcagagcctg gagaagtctc
     7801 aggatgtctc agttcacatc accagtaatt atctcaaaca gatcttaaat gctgcctatc
     7861 atgttgaagt cacgtttcac tcagggtcgt cagttacaag gatgctatgg gagcaaatca
     7921 aacagatcat ccagcgcatc acatgggtga gcccacctgc catcactctg gaatggaaga
     7981 ggaaggtggc ccaggaagcc attgagagcc tcagcgcctc caaattggct aagagcattt
     8041 gcagccaatt ccggactcgg ctcaatagtt cccacgaggc ttttgcagcc tccttgcggc
     8101 agctggaagc tggccactca ggccggttag agaaaacgga agatctatgg ctgagggttc
     8161 ggaaagatca tgctccccgc ctggcccgcc tttctctgga aagctgttct ttacaggatg
     8221 tcttgcttca tcgtaaacct aaactgggac aggaactggg ccggggccag tatggtgtgg
     8281 tatacctgtg tgacaactgg ggaggacact tcccttgtgc cctcaaatca gttgtccctc
     8341 cagatgagaa gcactggaat gatctggctt tggaatttca ctatatgagg tctctgccga
     8401 agcatgagcg attggtggat ctccatggtt cagtcattga ctacaactat ggtggtggct
     8461 ccagcattgc tgtgctcctc attatggagc ggctacaccg ggatctctac acagggctga
     8521 aggctgggct gaccctggag acacgtttgc agatagcact agatgtggtg gagggaatcc
     8581 gcttcctgca cagccaggga cttgtccatc gtgatatcaa actgaaaaat gtgctgctgg
     8641 ataagcagaa ccgtgccaag atcactgact taggattctg caagccagag gccatgatgt
     8701 caggcagcat tgtggggaca ccaatccata tggcccctga acttttcaca gggaagtacg
     8761 ataattccgt ggatgtctac gcttttggaa ttcttttctg gtatatctgc tcaggctctg
     8821 tcaagctccc tgaggcattt gagaggtgtg ctagcaaaga ccatctctgg aacaatgtgc
     8881 ggaggggggc tcgcccagaa cgtcttcctg tgtttgatga ggagtgctgg cagttgatgg
     8941 aagcctgttg ggatggcgac cccttgaaga ggcctctctt gggcattgtc cagcccatgc
     9001 tccagggcat catgaatcgg ctctgcaagt ccaattctga gcagccaaac agaggactag
     9061 atgattctac ttaggaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg
     9121 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     9181 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9241 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9301 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9361 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9421 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9481 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9541 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9601 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9661 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     9721 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     9781 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     9841 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     9901 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     9961 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    10021 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    10081 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    10141 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    10201 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
    10261 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    10321 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
    10381 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    10441 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    10501 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    10561 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    10621 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    10681 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    10741 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    10801 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
    10861 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
    10921 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10981 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
    11041 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctgcc tagggaaccc
    11101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11221 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    11281 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11401 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
    11461 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    11521 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    11581 gtcgagacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
    11641 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    11701 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    11761 agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg
    11821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    11881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    12181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
    12241 caatttcc
//
