LOCUS       sequence-305581-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of DSTYK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DSTYK _IDG-K
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..136)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..919
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWXGGEISLRSAEK"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acggccaact
      121 tttgtataca aagtgggcca ccatgggaat cccgctgctg aacgctaaac agattctggg
      181 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
      241 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
      301 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
      361 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
      421 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
      481 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
      541 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
      601 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
      661 gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
      721 ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc
      781 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
      841 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggntgggcg gagaaatcag
      901 tctgcggtct gccgaaaagg ctagcggcaa
//
