LOCUS       sequence-305582-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of DSTYK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-DSTYK _IDG-K
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
       61 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
      121 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatgg agggcgacgg
      181 ggtgccatgg ggcagcgagc ccgtctcggg tcccggcccc ggcggcggcg gaatgatccg
      241 cgagctgtgc cggggcttcg gccgctaccg ccgctacctg ggacggctgc gacagaacct
      301 gcgcgagacc cagaagttct tccgcgacat caagtgctcc cacaaccaca cttgtctctc
      361 ctccctcacg ggcggcggcg gggccgagcg cggccctgca ggcgatgtcg ccgaaaccgg
      421 gctgcaggcg ggccaactga gctgcatttc cttcccacct aaggaagaga agtacctcca
      481 gcagattgtg gactgcctcc cttgcatact gatcctcggc caggattgta acgtcaagtg
      541 ccagctgttg aatctgctgt tgggggtgca ggtgcttccc accaccaagc tgggcagtga
      601 ggagagctgt aagcttcggc gcctccgctt cacctatggg actcagactc gggtcagcct
      661 ggcgctccct ggacagtatg aactagtgca cacgctggtt gctcatcagg gcaactggga
      721 gaccatccct gaggaggatc tggaggtcca agagaacaat gaggatgctg ttcatgtttt
      781 agcggaactg gaggtaacga tgcaccatgc tctcttacag gaagtggacg ttgtggtagc
      841 accatgccaa ggcctccggc ccacagtggn ngttctgggt gacttggnga atgattcttg
      901 cctgtgatna ncctatgcac tccacaaaga tgaactctct ga
//
